Aptamer regulated nucleic acids and uses thereof
First Claim
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1. A nucleic acid comprising (i) a substrate sequence that can form a substrate for an extrinsic enzymatic activity, and (ii) an aptamer that binds to a ligand, wherein binding of the ligand to said aptamer causes a conformational change in the nucleic acid that alters the ability of said substrate sequence to form said substrate and/or alters the Km and/or Kcat of said substrate for the extrinsic enzymatic activity.
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Abstract
The invention relates to trans-acting ligand-responsive nucleic acids and uses thereof. In particular, a ligand responsive nucleic acid comprises an effector domain and an aptamer domain that is responsive to a ligand.
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Citations
26 Claims
- 1. A nucleic acid comprising (i) a substrate sequence that can form a substrate for an extrinsic enzymatic activity, and (ii) an aptamer that binds to a ligand, wherein binding of the ligand to said aptamer causes a conformational change in the nucleic acid that alters the ability of said substrate sequence to form said substrate and/or alters the Km and/or Kcat of said substrate for the extrinsic enzymatic activity.
- 2. A nucleic acid comprising (i) an antisense sequence for inhibiting expression of a target gene, and (ii) an aptamer that binds to a ligand, wherein binding of the ligand to said aptamer causes a conformational change in the nucleic acid that alters the ability of said antisense sequence to inhibit expression of the target sequence.
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3. A nucleic acid comprising (i) a hybridization sequence that hybridizes to a target sequence of a second nucleic acid, and (ii) an aptamer that binds to a ligand, wherein binding of the ligand to said aptamer causes a conformational change in the nucleic acid that alters the ability of said hybridization sequence to hybridize to said target sequence.
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4. A trans-acting nucleic acid for altering expression of a target gene, comprising:
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(i) a targeting sequence that is capable of hybridizing to said target gene and regulate expression of said target gene; and
(ii) an aptamer sequence that binds to a ligand, wherein, binding of said ligand to said aptamer causes a conformational change in said nucleic acid, and said nucleic acid regulates expression of said target gene in a manner dependent on the presence of said ligand. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 20, 22, 26)
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23. A method for rendering expression of a target gene in a cell dependent on the presence or absence of a ligand, comprising introducing into the cell a trans-acting nucleic acid comprising:
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(i) a targeting sequence that is capable of hybridizing to said target gene and regulate expression of said target gene; and
(ii) an aptamer sequence that binds to a ligand, wherein binding of said ligand to said aptamer induces a conformational change in said trans-acting nucleic acid, and said trans-acting nucleic acid regulates expression of said target gene in a dose-dependent manner dependent on the concentration of said ligand. - View Dependent Claims (24, 25)
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Specification