Method and apparatus for investigating histology of epithelial tissue
First Claim
1. A method for monitoring the presence of selected chromophores in a sample of epithelial tissue, independent of the amount of a predetermined chromophore, the method comprising:
- illuminating an area of tissue by projecting light from a light source of at least two different wavelengths λ
1, λ
2;
receiving light remitted by the illuminated area of tissue at a photoreceptor;
analysing the received light to obtain a measurement Rt(λ
) for each wavelength and then calculating;
where l is chosen such that Z is independent of the amount of predetermined chromophore.
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Abstract
A method for monitoring the presence of selected chromophores in a sample of epithelial tissue, independent of the amount of a predetermined chromophore, the method comprising: illuminating an area of tissue by projecting light from a light source of at least two different wavelengths λ1, λ2; receiving light remitted by the illuminated area of tissue at a photoreceptor; analysing the received light to identify and measure the proportion of light of each wavelength remitted from the tissue Ir (λ); calculating the ratio of light at each wavelength returned from the tissue Rt, (λ), and then calculating Z=Formula (I); where 1 is chosen such that Z is independent of the amount of predetermined chromophore. Typically 1 is calculated such that Z=Formula (II); where j and k are such that 2j α(λ1)=2kj α(λ2)=1 where α(λ1) and α(λ2) are the absorbtion coefficients for the predetermined chromophore at each wavelength.
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Citations
16 Claims
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1. A method for monitoring the presence of selected chromophores in a sample of epithelial tissue, independent of the amount of a predetermined chromophore, the method comprising:
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illuminating an area of tissue by projecting light from a light source of at least two different wavelengths λ
1, λ
2;
receiving light remitted by the illuminated area of tissue at a photoreceptor;
analysing the received light to obtain a measurement Rt(λ
) for each wavelength and then calculating;
where l is chosen such that Z is independent of the amount of predetermined chromophore. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method of forming an image of an area of epithelial tissue independent of the amount of a predetermined chromophore in the tissue, locations, formed by obtaining Z for a plurality of locations within the area, Z being obtained by illuminating an area of tissue by projecting light from a light source of at least two different wavelengths λ
-
1, λ
2;
receiving light remitted by the illuminated area of tissue at a photoreceptor;
analysing the received light to analysing the received light to obtain a measurement Rt(λ
) for each wavelength and then calculating;
where l is chosen such that Z is independent of the amount of predetermined chromophore; and
mapping the amounts Z at positions indicative of the location within the area of the measurement. - View Dependent Claims (11, 12, 13, 14)
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1, λ
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15. Apparatus for monitoring the presence of selected chromophores in a sample of epithelial tissue, independent of the amount of a predetermined chromophore comprising a light source for illuminating tissue with light of at least two different wavelengths λ
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1, λ
2.a photoreceptor for receiving images remitted by the illuminated area of tissue at a photoreceptor; and
microprocessor means for analysing the received light to identify and measure the proportion of light of each wavelength remitted from the tissue Ir(λ
);
calculating the ratio of light at each wavelength returned from the tissue Rt(λ
), and then calculating;
where l is chosen such that Z is independent of the amount of predetermined chromophore. - View Dependent Claims (16)
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1, λ
Specification