Device including a dissolvable structure for flow control

• US 20060093528A1
• Filed: 10/18/2005
• Published: 05/04/2006
• Est. Priority Date: 10/18/2004
• Status: Abandoned Application

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applera Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Faulstich, Konrad, Ulmanella, Umberto, Xie, Jun, Lau, Aldrich N. K., Banerjee, Debjyoti
• Application Number:
  US11/252,821
• Publication Number:
  US 20060093528A1
• Time in Patent Office: Days
• US Class Current: 422/400
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0655 pinch valves
• CPC Class Codes: B01L 2400/0661 shape memory polymer valves
• CPC Class Codes: B01L 2400/0677 phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
• CPC Class Codes: B01L 2400/0683 mechanically breaking a wall or membrane within a channel or chamber
• CPC Class Codes: B01L 2400/082 Active control of flow resistance, e.g. flow controllers
• CPC Class Codes: B01L 2400/084 Passive control of flow resistance
• CPC Class Codes: B01L 2400/086 using baffles or other fixed flow obstructions
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: F16K 2099/0084 Chemistry or biology, e.g. "lab-on-a-chip" technology
• CPC Class Codes: F16K 99/0001 Microvalves microdevices B81B1/00; manufacture or treatment of devices or systems in or on a substrate B81C1/00; microfluidic structures B01L3/5027; micropumps F04B19/006
• CPC Class Codes: F16K 99/003 : Valves for single use only
• CPC Class Codes: F16K 99/0036 : operated by temperature variations
• CPC Class Codes: F16K 99/0057 : the fluid being the circulating fluid itself, e.g. check valves
• CPC Class Codes: Y10S 422/901 : Polymer dissolver
• CPC Class Codes: Y10T 137/0318 : Processes
• CPC Class Codes: Y10T 137/0324 : With control of flow by a condition or characteristic of a fluid
• CPC Class Codes: Y10T 137/1624 : Destructible or deformable element controlled
• CPC Class Codes: Y10T 137/1812 : In fluid flow path
• CPC Class Codes: Y10T 137/2191 : By non-fluid energy field affecting input [e.g., transducer]
• CPC Class Codes: Y10T 137/8376 : Combined
• CPC Class Codes: Y10T 137/8593 : Systems
• CPC Class Codes: Y10T 137/85978 : With pump
• CPC Class Codes: Y10T 436/143333 : Saccharide [e.g., DNA, etc.]
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

A diagnostic device is provided that includes a plurality of retainment regions, with the retainment regions that are separated by at least one dissolvable barrier. The retainment regions can be interconnected through at least one fluid processing passageway. A retainment region can include a container such as a retainment region, well, chamber, or other receptacle, or a retainment region such as a surface on which the material is retained. The retainment regions can include a reaction retainment region, one or more reagent retainment regions, each containing unreacted reagents, and a sample retainment region. A pressure-actuated valve can be positioned in each fluid processing passageway interconnecting the one or more reagent retainment regions with the respective intermediate retainment regions interposed between each of the one or more reagent retainment regions and the reaction retainment region. The dissolvable barrier can be a fluid flow modulator in the at least one fluid processing passageway.

Claims

1. A fluid processing device comprising:

a fluid processing passageway;

a plurality of retainment regions, at least two of the retainment regions each being in fluid communication with the fluid processing passageway; and

at least one fluid flow modulator arranged in the fluid processing passageway and being adapted to open to form, or to increase in size, a fluid communication between the at least two retainment regions, the fluid flow modulator comprising at least one of a polyethylene glycol material, a derivative of a polyethylene glycol material, and a combination thereof, and being adapted to dissolve when contacted with water at room temperature.

2. The fluid processing device of claim 1, wherein at least one of the plurality of retainment regions comprises aqueous fluid retained therein.

3. The fluid processing device of claim 1, wherein the at least one fluid flow modulator comprises at least one of a polyethylene glycol material and a derivative of a polyethylene glycol material, having a melting point of from about 35°

C. and about 65°

C.

4. The fluid processing device of claim 1, wherein the fluid processing passageway is dimensioned sufficient to cause capillary flow of a fluid from at least one of the at least two retainment regions through the fluid processing passageway.

5. The fluid processing device of claim 1, wherein the fluid processing passageway is dimensioned sufficient to cause electrokinetic migration of charged components in a fluid, from at least one of the at least two retainment regions through the fluid processing passageway.

6. The fluid processing device of claim 5, further comprising at least two electrodes disposed in the device with the fluid processing passageway therebetween.

7. The fluid processing device of claim 1, further comprising:

at least one additional retainment region;

at least one additional fluid processing passageway; and

at least one valve comprising one or more of a pressure-actuatable valve and a heat-actuatable valve arranged in the at least one additional fluid processing passageway, wherein the at least one additional fluid processing passageway is in fluid communication with the at least one additional retainment region and at least one of the plurality of retainment regions.

8. A system comprising the fluid processing device of claim 1, and a pump, wherein the pump is arranged in fluid communication with at least one of the fluid processing passageway and one or more of the plurality of retainment regions.

9. A system comprising the fluid processing device of claim 6, a power source, and at least two electrical leads forming electrical connections, respectively, between the power source and the at least two electrodes.

10. The fluid processing device of claim 1, wherein the at least one fluid flow modulator comprises a material having the formula:

R₁—

Q-(—

CH₂—

)_p-(—

OCH₂CH₂—

)_m-(—

CH₂—

)_q-G—

R₂ 

 

Formula 1

wherein

G and Q are each independently a single bond, O, N,

R₁ and R₂ are each independently H, OH, NH₂, O(C_nH_2n+1), O(C_nH<sub>2n−

1</sub>), CH₂OH,

-(—

CH₂CH₂O—

)_n—

H, CH₂CH₂CH₂NH₂, CH₂CO₂H, C_gH<sub>2g−

1</sub>, or C_nH_2n+1,

R₉, R₁₀, R₁₁, R₁₂, R₁₃, and R₁₄, are each independently O, S, or NH,

p and q are each independently 0, 1, 2, or 3,

m is an integer from 0 to about 10,00,

at least one of p, q, and m is an integer greater than 0,

g is an integer from 2 to about 20, and

n is an integer from 1 to about 20;

R₄, R₅, and R₆ are each independently H, OH, NH₂, O(C_nH_2n+1), O(C_nH<sub>2n−

1</sub>), CH₂OH,

-(—

CH₂CH₂O—

)_n—

H, CH₂CH₂CH₂NH₂, CH₂CO₂H, C_gH<sub>2g−

1</sub>, or C_nH_2n+1,

u is an integer from 0 to about 10,000,

g is an integer from 2 to about 20,

n is an integer from 1 to about 20,

t, v, and z are each independently an integer from 0 to about 10,000, and

at least one of t, u, and v, is an integer greater than 0;

[R₇-(—

CH₂CH₂O—

)_x-(—

CH₂CH₂—

)_r-]a-A—

R₃—

B-[-(—

CH₂CH₂—

)_s-(—

CH₂CH₂O—

)_y—

R₈]_b 

 

Formula 3

wherein

A and B are each independently a single bond, O, N,

R₇ and R₈ are each independently H, OH, NH₂, O(C_nH₂₊₁), O(C_nH<sub>2n−

1</sub>), CH₂OH,

-(—

CH₂CH₂O—

)_n—

H, CH₂CH₂CH₂NH₂, CH₂CO₂H, C_gH<sub>2g−

1</sub>, or C_nH_2n+1,

R₃ is C_nH_2n, C_nH<sub>2n−

2</sub>, or CH₂CH(CH₃)O,

R₉, R₁₀, R₁₁, R₁₂, R₁₃, and R₁₄, can each independently be O, S, or NH,

a, b, r, and s are each independently 0, 1, 2, or 3,

x and y are each independently an integer from 1 to about 10,000,

g is an integer from 2 to about 20, and

n is an integer from 1 to about 20;

or

wherein;

A, G, and Q are each independently a single bond, O, N,

R₁, R₂, R₄, and R₅ are each independently H, OH, NH₂, O(C_nH_2n+1), O(C_nH<sub>2n−

1</sub>), CH₂OH,

-(—

CH₂CH₂O—

)_n—

H, CH₂CH₂CH₂NH₂, CH₂CO₂H, C_gH<sub>2g−

1</sub>, or C_nH_2n+1,

R₉, R₁₀, R₁₁, R₁₂, R₁₃, and R₁₄, are each independently O, S, or NH,

f is an integer from 1 to about 10,000,

p and q are each independently 0, 1, 2, or 3,

m is an integer from 0 to about 10,000,

at least one of p, q, and m is an integer greater than 0,

g is an integer from 2 to about 20, and

n is an integer from 1 to about 20.

11. A fluid processing device comprising:

a fluid processing passageway; and

at least one fluid flow modulator arranged in the fluid processing passageway and being adapted to open to form, or increase the size of, a fluid communication through the fluid processing passageway, the at least one fluid flow modulator being adapted to dissolve when contacted with water at room temperature, and having the formula;

R₁—

Q-(—

CH₂—

)_p-(—

OCH₂CH₂—

)_m-(—

CH₂—

)_q-G—

R₂ 

 

Formula 1

wherein

G and Q are each independently a single bond, O, N,

R₁ and R₂ are each independently H, OH, NH₂, O(C_nH_2n+1), O(C_nH<sub>2n−

1</sub>), CH₂OH,

-(—

CH₂CH₂O—

)_n—

H, CH₂CH₂CH₂NH₂, CH₂CO₂H, C_gH<sub>2g−

1</sub>, or C_nH_2n+1,

R₉, R₁₀, R₁₁, R₁₂, R₁₃, and R₁₄, are each independently O, S, or NH,

p and q are each independently 0, 1, 2, or 3,

m is an integer from 0 to about 10,000,

at least one of p, q, and m is an integer greater than 0,

g is an integer from 2 to about 20, and

n is an integer from 1 to about 20;

wherein

R₄, R₅, and R₆ are each independently H, OH, NH₂, O(C_nH_2n+1), O(C_nH<sub>2n−

1</sub>), CH₂OH,

-(—

CH₂CH₂O—

)_n—

H, CH₂CH₂CH₂NH₂, CH₂CO₂H, C_gH<sub>2g−

1</sub>, or C_nH_2n+1,

u is an integer from 0 to about 10,000,

g is an integer from 2 to about 20,

n is an integer from 1 to about 20,

t, v, and z are each independently an integer from 0 to about 10,000, and

at least one of t, u, and v, is an integer greater than 0;

[R₇-(—

CH₂CH₂O—

)_x-(—

CH₂CH₂—

)_r-]_a-A—

R₃—

B-[-(—

CH₂CH₂—

)_s-(—

CH₂CH₂O—

)_y—

R₈]_b 

 

Formula 3

wherein

A and B are each independently a single bond, O, N,

R₇ and R₈ are each independently H, OH, NH₂, O(C_nH_2n+1), O(C_nH<sub>2n−

1</sub>), CH₂OH,

-(—

CH₂CH₂O—

)_n—

H, CH₂CH₂CH₂NH₂, CH₂CO₂H, C_gH<sub>2g−

1</sub>, or C_nH_2n+1,

R₃ is C_nH_2n, C_nH<sub>2n−

2</sub>, or CH₂CH(CH₃)O,

R₉, R₁₀, R₁₁, R₁₂, R₁₃, and R₁₄, can each independently be O, S, or NH,

a, b, r, and s are each independently 0, 1, 2, or 3,

x and y are each independently an integer from 1 to about 10,000,

g is an integer from 2 to about 20, and

n is an integer from 1 to about 20;

or

wherein

A, G, and Q are each independently a single bond, O, N,

R₁, R₂, R₄, and R₅ are each independently H, OH, NH₂, O(C_nH_2n+1), O(C_nH<sub>2n−

1</sub>), CH₂OH,

-(—

CH₂CH₂O—

)_n—

H, CH₂CH₂CH₂NH₂, CH₂CO₂H, C_gH<sub>2g−

1</sub>, C_nH_2n+1, (C_nH_2n+1)(CN)₂C, or

SO₄H;

R₉, R₁₀, R₁₁, R₁₂, R₁₃, and R₁₄, are each independently O, S, or NH,

f is an integer from 1 to about 10,000,

p and q are each independently 0, 1, 2, or 3,

m is an integer from 0 to about 10,000,

at least one of p, q, and m is an integer greater than 0,

g is an integer from 2 to about 20, and

n is an integer from 1 to about 20.

12. A fluid processing device comprising:

a substrate;

a plurality of retainment regions formed in or on the substrate, comprising at least a first retainment region and a second retainment region; and

a barrier at least partially separating the first retainment region from the second retainment region, wherein said barrier comprises at least one of a polyethylene glycol material, a derivative of a polyethylene glycol material, and a combination thereof, and being adapted to dissolve when contacted with water at room temperature.

13. The fluid processing device claim 12, wherein said barrier completely separates the first retainment region from fluid communication with the second retainment region.

14. The fluid processing device claim 12, wherein the barrier comprises at least one of a polyethylene glycol material and a derivative of a polyethylene glycol material, having a molecular weight of from about 500 Daltons to about 5,000,000 Daltons.

15. The fluid processing device of claim 12, further comprising:

at least one additional retainment region;

at least one fluid processing passageway; and

at least one valve comprising a pressure-actuatable valve or a heat-actuatable valve arranged in the at least one fluid processing passageway, wherein the at least one fluid processing passageway is in fluid communication with the at least one additional retainment region and at least one of the plurality of retainment regions.

16. The fluid processing device of claim 15, wherein the at least one valve comprises at least one pressure-actuatable valve comprising a burstable valve that is adapted to open and establish fluid communication only upon receiving pressure of at least about 1 psig from a fluid in the at least one additional retainment region.

17. The fluid processing device of claim 12, wherein the barrier comprises one or more of a substituted polyethylene glycol material, a polyethylene glycol derivative, and a branched polyethylene glycol, a derivative of a branched polyethylene glycol, and a combination thereof.

18. The fluid processing device of claim 12, wherein the barrier comprises a material having the formula:

R₁—

Q-(—

CH₂—

)_p-(—

OCH₂CH₂—

)_m-(—

CH₂—

)_q-G—

R₂ 

 

Formula 1

wherein

G and Q are each independently a single bond, O, N,

R₁ and R₂ are each independently H, OH, NH₂, CH₃, C₂H₅, OCH₃, OC₂H₅, CH₂OH,

-(—

CH₂CH₂O—

)_n—

H, CH₂CH₂CH₂NH₂, CH₂CO₂H, C_gH<sub>2g−

1</sub>, or C_nH_2n+1,

R₉, R₁₀, R₁₁, R₁₂, R₁₃, and R₁₄, are each independently O, S, or NH,

p and q are each independently 0, 1, 2, or 3,

m is an integer from 0 to about 10,000;

at least one of p, q, and m is an integer greater than 0,

g is an integer from 2 to about 20, and

n is an integer from 1 to about 20;

wherein

R₄, R₅, and R₆ are each independently H, OH, NH₂, CH₃, C₂H₅, OCH₃, OC₂H₅, CH₂OH,

-(—

CH₂CH₂O—

)_n—

H, CH₂CH₂CH₂NH₂CH₂CO₂H, C_gH<sub>2g−

1</sub>, or C_nH_2n+1,

u is an integer from 0 to about 10,000,

g is an integer from 2 to about 20,

n is an integer from 1 to about 20,

t, v, and z are each independently an integer from 0 to about 10,000, and

at least one of t, u, and v, is an integer greater than 0;

[R₇-(—

CH₂CH₂O—

)_x-(—

CH₂CH₂—

)_r-]_a-A—

R₃—

B-[-(—

CH₂CH₂—

)_s-(—

CH₂CH₂O—

)_y—

R₈]_b 

 

Formula 3

wherein

A and B are each independently a single bond, O, N,

R₇ and R₈ are each independently H, OH, NH₂, CH₃, C₂H₅, OCH₃, OC₂H₅, CH₂OH,

-(—

CH₂CH₂O—

)_n—

H, CH₂CH₂CH₂NH₂, CH₂CO₂H, C_gH<sub>2g−

1</sub>, or C_nH_2n+1,

R₃ is C_nH_2n, C_nH<sub>2n−

2</sub>, or CH₂CH(CH₃)O,

R₉, R₁₀, R₁₁, R₁₂, R₁₃, and R₁₄, can each independently be O, S, or NH,

a, b, r, and s are each independently 0, 1, 2, or 3,

x and y are each independently an integer from 1 to about 10,000,

g is an integer from 2 to about 20, and

n is an integer from 1 to about 20;

or

wherein;

A, G, and Q are each independently a single bond, O, N,

R₁, R₂, R₄, and R₅ are each independently H, OH, NH₂, CH₃, C₂H₅, OCH₃, OC₂H₅, CH₂OH,

-(—

CH₂CH₂O—

)_n—

H, CH₂CH₂CH₂NH₂, CH₂CO₂H, C_gH<sub>2g−

1</sub>, or C_nH_2n+1,

R₉, R₁₀, R₁₁, R₁₂, R₁₃, and R₁₄, are each independently O, S, or NH,

f is an integer from 1 to about 10,000,

p and q are each independently 0, 1, 2, or 3

m is an integer from 0 to about 10,000,

at least one of p, q, and m is an integer greater than 0,

g is an integer from 2 to about 20, and

n is an integer from 1 to about 20.

19. A method comprising:

providing a fluid processing device comprising at least a first retainment region and a second retainment region, and a barrier arranged between the first retainment region and the second retainment region, wherein at least one of the first and second retainment regions retains an aqueous solution, the barrier comprises at least one of a polyethylene glycol material, a derivative of a polyethylene glycol material, and a combination thereof, and the barrier is adapted to dissolve when contacted with the aqueous solution; and

contacting the barrier with the aqueous solution to dissolve at least a portion of the barrier and form, or increase the size of, a fluid communication between the first retainment region and the second retainment region.

20. A method of performing a set of predetermined assays, comprising:

providing a plurality of retainment regions in a closed, disposable cuvette, the retainment regions interconnected by fluid processing passageways but closed to fluid flow to or from locations outside of the cuvette, the cuvette including;

first retainment regions;

second retainment regions;

first fluid processing passageways interconnecting the first retainment regions from the second retainment regions, wherein the first retainment regions are selectively closed-off from fluid communication with the second retainment regions by pressure-actuated valves positioned in the first fluid processing passageways;

one or more third retainment regions interconnected by second fluid processing passageways with at least the second retainment regions; and

fluid flow modulators positioned in the second fluid processing passageways;

applying pressure to the pressure actuated valves in the first fluid processing passageways sufficient to provide fluid communication between the first and second retainment regions;

introducing a sample for testing or other processing into the one or more third retainment regions; and

establishing fluid communication between the second retainment regions and the one or more third retainment regions at a controlled rate that is a function of characteristics of at least one of the sample in the one or more third retainment regions and a fluid within the second retainment regions.