Olfactory receptor expression libraries and methods of making and using them
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Abstract
This invention provides novel libraries of olfactory receptor odorant/ligand-binding domains and methods of making and using them. The invention also provides libraries of vectors and cells comprising these nucleic acid constructs. The compositions and methods of the invention are used to identify novel ligand-binding domains for olfactory neuron odorant receptors and their ligands. Thus, the compositions and methods of the invention can be used to generate novel odorants, to screen for toxic odorants, or to manipulate an animal'"'"'s olfactory response.
6 Citations
52 Claims
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1-51. -51. (canceled)
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52. A nucleic acid construct that facilitates the translocation of a G protein coupled receptor (GPCR) across a cell membrane, said construct comprising:
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(i) a first nucleic acid sequence that encodes the rhodopsin translocation peptide contained in SEQ ID NO;
3 or a nucleic acid sequence encoding a variant of said rhodopsin translocation peptide, wherein said variant is identical to said rhodopsin translocation peptide but for conservative amino acid substitution modifications; and
(ii) a second nucleic acid sequence that encodes a heterologous GPCR, wherein said nucleic acid construct when expressed in a cell results in a hybrid polypeptide comprising the rhodopsin translocation peptide and the heterologous GPCR, wherein the rhodopsin translocation peptide directs the translocation of the heterologous GPCR across the cell membrane.
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Specification