Ammonium transporter promoter for gene expression in oleaginous yeast
First Claim
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1. A method for the expression of a coding region of interest in a transformed yeast comprising:
- c) providing a transformed yeast having a genetic construct comprising;
(i) a promoter region of a Yarrowia yat1 gene; and
, (ii) a coding region of interest expressible in the yeast cell;
wherein the promoter region is operably linked to the coding region of interest; and
, d) growing the transformed yeast of step (a) under conditions whereby the genetic construct of step (a) is expressed.
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Abstract
The promoter region associated with the Yarrowia lipolytica ammonium transporter (yat1) gene has been found to be particularly effective for the expression of heterologous genes in oleaginous yeast. The promoter regions of the instant invention have been shown to be advantageously inducible under oleaginous conditions (i.e., nitrogen limitation) and are useful to drive expression of genes involved in the production of ω-3 and ω-6 fatty acids.
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23 Claims
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1. A method for the expression of a coding region of interest in a transformed yeast comprising:
-
c) providing a transformed yeast having a genetic construct comprising;
(i) a promoter region of a Yarrowia yat1 gene; and
,(ii) a coding region of interest expressible in the yeast cell;
wherein the promoter region is operably linked to the coding region of interest; and
,d) growing the transformed yeast of step (a) under conditions whereby the genetic construct of step (a) is expressed. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method for the production of an ω
- -3 or an ω
-6 fatty acid comprising;
d) providing a transformed oleaginous yeast comprising a genetic construct, comprising;
(i) a promoter region of a Yarrowia yat1 gene; and
,(ii) a coding region encoding at least one enzyme of the ω
-3/ω
-6 fatty acid biosynthetic pathway;
wherein the promoter region and coding region are operably linked;
e) culturing the transformed oleaginous yeast of step (a) under conditions whereby the at least one enzyme of the ω
-3/ω
-6 fatty acid biosynthetic pathway is expressed and a ω
-3 or ω
-6 fatty acid is produced; and
,f) optionally recovering the ω
-3 or ω
-6 fatty acid. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
- -3 or an ω
Specification