Integration of sample storage and sample management for life science
First Claim
Patent Images
1. A matrix for substantially dry storage of a biological sample, comprising:
- (a) a matrix material that dissolves or dissociates in a solvent; and
(b) at least one stabilizer, wherein the stabilizer is not lactitol, lactose, maltose, maltitol, mannitol, sucrose, sorbitol, cellobiose, inositol or chitosan, and wherein if the at least one stabilizer comprises a first stabilizer that is trehalose, then a trehalase inhibitor is also present as a second stabilizer.
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Abstract
Compositions and methods are disclosed for automated storing, tracking, retrieving and analyzing biological samples, including dry storage at ambient temperatures of nucleic acids, proteins (including enzymes), and cells using a dissolvable dry storage matrix that permits recovery of biologically active materials. RFID-tagged biological sample storage devices featuring dissolvable or dissociable matrices are described for use as supports of biological samples, which matrices can be dried and subsequently rehydrated for sample recovery. Also disclosed are computer-implemented systems and methods for managing sample data.
172 Citations
67 Claims
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1. A matrix for substantially dry storage of a biological sample, comprising:
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(a) a matrix material that dissolves or dissociates in a solvent; and
(b) at least one stabilizer, wherein the stabilizer is not lactitol, lactose, maltose, maltitol, mannitol, sucrose, sorbitol, cellobiose, inositol or chitosan, and wherein if the at least one stabilizer comprises a first stabilizer that is trehalose, then a trehalase inhibitor is also present as a second stabilizer. - View Dependent Claims (8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39)
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2. A matrix for substantially dry storage of a biological sample, comprising:
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(a) a matrix material that dissolves or dissociates in a solvent; and
(b) at least two stabilizers, wherein the stabilizer is not lactitol, lactose, maltose, maltitol, mannitol, sucrose, sorbitol, cellobiose, inositol or chitosan, and wherein if one of the at least two stabilizers comprises a first stabilizer that is trehalose, then a trehalase inhibitor is also present as a second stabilizer.
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3. A matrix for substantially dry storage of a biological sample, comprising:
(a) a matrix material that dissolves or dissociates in a solvent;
(b) at least one stabilizer; and
(c) at least one biological sample, wherein the stabilizer is not lactitol, lactose, maltose, maltitol, mannitol, sucrose, sorbitol, cellobiose, inositol or chitosan, and wherein if the at least one stabilizer comprises a first stabilizer that is trehalose, then a trehalase inhibitor is also present as a second stabilizer.- View Dependent Claims (25)
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4. A matrix for substantially dry storage of a biological sample, comprising:
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(a) a matrix material that dissolves or dissociates in a solvent, said matrix material comprising polyvinyl alcohol; and
(b) at least one stabilizer.
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5. A matrix for substantially dry storage of a biological sample, comprising:
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(a) a matrix material that dissolves or dissociates in a solvent; and
(b) at least one stabilizer, wherein said at least one stabilizer comprises a trehalase inhibitor.
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6. A matrix for substantially dry storage of a biological sample, comprising:
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(a) a matrix material that dissolves or dissociates in a solvent; and
(b) at least one and no more than two stabilizers, wherein the stabilizer is not trehalose, lactitol, lactose, maltose, maltitol, mannitol, sucrose, sorbitol, cellobiose, inositol or chitosan.
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7. A matrix for substantially dry storage of a biological sample, comprising:
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(a) a matrix material that dissolves or dissociates in a solvent; and
(b) at least one stabilizer, wherein said at least one stabilizer comprises a glycosidase inhibitor that is selected from the group consisting of;
(i) a trehalase inhibitor, (ii) a chitinase inhibitor, (iii) an α
-glucosidase inhibitor,(iv) a glycogen phosphorylase inhibitor, (vi) a neuramimidase inhibitor, (vi) a ceramide glucosyltransferase inhibitor, and (vii) a lysosomal glycosidase inhibitor.
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26. A matrix for substantially dry storage of a biological sample, comprising:
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(a) a matrix material that dissolves or dissociates in a solvent, said matrix material comprising polyvinyl alcohol; and
(b) a first stabilizer which comprises trehalose; and
(c) a second stabilizer which comprises validamycin A.
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40. A matrix for substantially dry storage of a biological sample, comprising:
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(a) at least one matrix material comprising a polymer that dissolves or dissociates in a solvent; and
(b) at least one stabilizer, wherein the stabilizer is not lactitol, lactose, maltose, maltitol, mannitol, sucrose, sorbitol, cellobiose, inositol or chitosan, and wherein if the at least one stabilizer comprises a first stabilizer that is trehalose, then a trehalase inhibitor is also present as a second stabilizer, wherein;
(I) the matrix material of (a) does not covalently self-assemble and has the structure;
-[—
X—
]n-wherein X is —
CH3, —
CH2—
, —
CH2CH(OH)—
, substituted —
CH2CH(OH)—
, —
CH2CH(COOH)—
, substituted —
CH2CH(COOH)—
, —
CH═
CH2, —
CH═
CH—
, C1-C24 alkyl or substituted alkyl, C2-24 alkenyl or substituted alkenyl, polyoxyethylene, polyoxypropylene, or a random or block copolymer thereof;
and wherein n is an integer having a value of about 1-100, 101-500, 501-1000, 1001-1500, or 1501-3000;
and wherein (II) the stabilizer is not covalently linked to the polymer and comprises trehalose, a trehalase inhibitor, or a compound comprising a structure that is selected from the group consisting of formulae (i)-(xv);
wherein R is selected from —
H, —
OH, —
CH2OH, —
NHAc and —
OAc. - View Dependent Claims (41, 42, 43)
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44. A method of storing a biological sample, comprising:
contacting a biological sample with a matrix for substantially dry storage of a biological sample, the matrix comprising (i) a matrix material that dissolves or dissociates in a solvent; and
(ii) at least one stabilizer, wherein the stabilizer is not lactitol, lactose, maltose, maltitol, mannitol, sucrose, sorbitol, cellobiose, inositol, or chitosan, and wherein if the at least one stabilizer comprises a first stabilizer that is trehalose, then a trehalase inhibitor is also present as a second stabilizer, and thereby storing said biological sample.- View Dependent Claims (45)
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46. A method of storing a biological sample, comprising:
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(a) contacting a biological sample with a matrix for substantially dry storage of a biological sample, the matrix comprising (i) a matrix material that dissolves or dissociates in a solvent; and
(ii) at least one stabilizer, wherein the stabilizer is not lactitol, lactose, maltose, maltitol, mannitol, sucrose, sorbitol, cellobiose, inositol, or chitosan, and wherein if the at least one stabilizer comprises a first stabilizer that is trehalose, then a trehalase inhibitor is also present as a second stabilizer; and
(b) drying the matrix, and thereby storing said biological sample. - View Dependent Claims (47, 48, 49, 50, 51, 52)
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53. A method of preparing a biological sample storage device for one or a plurality of biological samples, comprising:
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(a) administering a matrix to one or a plurality of sample wells of a biological sample storage device, wherein (1) said biological sample storage device comprises (i) a lid, and (ii) a sample plate comprising one or a plurality of sample wells that are capable of containing a biological sample, and wherein (2) the matrix comprises (i) a matrix material that dissolves or dissociates in a solvent; and
(ii) at least one stabilizer, wherein the stabilizer is not lactitol, lactose, maltose, maltitol, mannitol, sucrose, sorbitol, cellobiose, inositol, or chitosan, and wherein if the at least one stabilizer comprises a first stabilizer that is trehalose, then a trehalase inhibitor is also present as a second stabilizer; and
(b) drying one or more of the sample wells, and thereby preparing the biological sample storage device. - View Dependent Claims (54, 55, 56, 57, 58, 59, 60, 61)
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62. A method of recovering a stored biological sample, comprising:
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(a) contacting, simultaneously or sequentially and in either order in a biological sample storage device, one or a plurality of biological samples with a matrix for substantially dry storage of a biological sample, wherein (1) said biological sample storage device comprises (i) a lid, and (ii) a sample plate comprising one or a plurality of sample wells that are capable of containing the biological sample, wherein one or more of said wells comprises the matrix, and wherein (2) the matrix comprises (i) a matrix material that dissolves or dissociates in a solvent, and (ii) at least one stabilizer, wherein the stabilizer is not lactitol, lactose, maltose, maltitol, mannitol, sucrose, sorbitol, cellobiose, inositol, or chitosan, and wherein if the at least one stabilizer comprises a first stabilizer that is trehalose, then a trehalase inhibitor is also present as a second stabilizer;
(b) drying one or more of the sample wells;
(c) maintaining the biological sample storage device without refrigeration subsequent to the steps of contacting and drying; and
(d) resuspending or redissolving the biological sample in a second solvent, and therefrom recovering the stored biological sample. - View Dependent Claims (63, 64, 65)
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66. A matrix for substantially dry storage of a biological sample, comprising:
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(a) a matrix material that dissolves or dissociates in a solvent;
(b) at least one stabilizer; and
(c) a sample treatment composition. - View Dependent Claims (67)
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Specification