Mitochondrial biology expression arrays
First Claim
1. An array comprising at least two isolated nucleotide molecules, each molecule having a sequence capable of uniquely hybridizing to a nucleic acid molecule which is an expression product of a gene involved in mitochondrial biology.
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Abstract
This invention provides a library of genes involved in mitochondrial biology, arrays containing probes for genes involved in mitochondrial biology, methods for making such arrays, and methods of using such arrays. Genes and probe sequences involved in mitochondrial biology in humans and mice are provided. The arrays of this invention are useful for determining mitochondrial biology gene expression profiles. Mitochondrial biology gene expression profiles are useful for determining expression profiles diagnostic of physiological conditions; diagnosing physiological conditions; identifying biochemical pathways, genes, and mutations involved in physiological conditions; identify therapeutic agents useful for preventing and/or treating such physiological conditions; evaluating and/or monitoring the efficacy of such therapies, and creating and identifying animal models of human physiologic conditions. Arrays containing probes for all genes known to be involved in mitochondrial biology are provided, as well as arrays containing subsets of such probes.
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Citations
20 Claims
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1. An array comprising at least two isolated nucleotide molecules, each molecule having a sequence capable of uniquely hybridizing to a nucleic acid molecule which is an expression product of a gene involved in mitochondrial biology.
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2. An array comprising two or more isolated nucleic acid molecules or spots, each spot comprising a plurality of isolated nucleic acid molecules, each molecule having a sequence consisting essentially of a sequence selected from the group consisting of the sequences of human probe set #1, SEQ ID NOS:
- 1 to 994, or mouse probe set #2, SEQ ID NOS;
995 to 3040, and sequences having at least 70% homology to the foregoing sequences. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
- 1 to 994, or mouse probe set #2, SEQ ID NOS;
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14. A method of making an array comprising:
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a) providing a prepared substrate; and
b) printing two or more spots in known positions on said substrate, each spot comprising a plurality of isolated nucleic acid molecules, each molecule having a sequence consisting essentially of a sequence selected from the group consisting of human probe set #1, SEQ ID NOS;
1 to 994, mouse probe set #2, SEQ ID NOS;
995 to 3040, and sequences having at least 70% homology to the foregoing sequences. - View Dependent Claims (15, 16)
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17. A method of diagnosing a first individual with Complex IV Leigh'"'"'s Syndrome comprising detecting in a first sample from said first individual at least about a 1.7-fold decrease in the amount of expression of genes comprising ND4, NDL4, ND6, SURF-1, SOD2, 70 kD heat shock protein, VDAC4, ANT2, and glutathione peroxidase 3 compared to the amount of expression of said genes in a second sample from a second individual without Complex IV Leigh'"'"'s Syndrome.
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18. A library of at least two isolated nucleic acid molecules, each molecule having a sequence consisting essentially of a sequence selected from the group consisting of human probe set #1, SEQ ID NOS:
- 1 to 994, mouse probe set #2, SEQ ID NOS;
995 to 3040, and sequences having at least 70% homology to the foregoing sequences.
- 1 to 994, mouse probe set #2, SEQ ID NOS;
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19. An array comprising at least two spots, each spot comprising a plurality of isolated nucleic acid molecules, each molecule comprising a sequence with at least 70% homology to a sequence selected from the group consisting of human probe set #1, SEQ ID NOS:
- 1 to 994.
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20. An array comprising at least two spots, each spot comprising a plurality of isolated nucleic acid molecules, each molecule comprising a sequence with at least 70% homology to a sequence selected from the group consisting of mouse probe set #2, SEQ ID NOS:
- 995 to 3040.
Specification