Method and apparatus for assay for multiple analytes
First Claim
1. A sensing element for use in a light diffraction assay for detecting the presence or absence of at least two analytes, comprising:
- a substrate including a surface and on said surface a first pre-selected pattern of first analyte-specific receptors and at least a second pre-selected pattern including second analyte-specific receptors, wherein each of said pre-selected patterns on said surface is distinct and, when bound to an analyte, gives rise to a pre-selected diffraction pattern distinct from diffraction patterns formed from all other unbound and bound pre-selected patterns on the surface.
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Abstract
A method and apparatus for assay of multiple analytes. The method uses a sensing element comprising a substrate upon which is arranged a multiplicity of recognition elements, such that each element is laid out in a predetermined pattern. Each pattern is unique in that it can give rise to a characteristic diffraction pattern in the assay. The patterns may or may not be interpenetrating on the substrate surface. The method of detecting multiple analytes includes contacting the medium of analytes with the patterned substrate, illuminating the substrate by a light source, and detecting any resultant diffraction image. The pattern of diffraction and the intensity of the diffracted signal provides information about the existence of specific analytes and their quantification.
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Citations
65 Claims
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1. A sensing element for use in a light diffraction assay for detecting the presence or absence of at least two analytes, comprising:
a substrate including a surface and on said surface a first pre-selected pattern of first analyte-specific receptors and at least a second pre-selected pattern including second analyte-specific receptors, wherein each of said pre-selected patterns on said surface is distinct and, when bound to an analyte, gives rise to a pre-selected diffraction pattern distinct from diffraction patterns formed from all other unbound and bound pre-selected patterns on the surface. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14-33. -33. (canceled)
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34. An apparatus for detection of analytes in a medium using diffraction of light, comprising:
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a source of illumination;
a sensing element including a substrate having a surface and on said surface a first pre-selected pattern of first analyte-specific receptors and at least a second pre-selected pattern including second analyte-specific receptors, wherein each of said pre-selected patterns on said surface is distinct and, when bound to an analyte, gives rise to a pre-selected diffraction pattern distinct from diffraction patterns formed from all other unbound and bound pre-selected patterns on the surface, said source of illumination being positioned so as to illuminate said substrate surface;
detection means positioned with respect to said sensing element to detect light diffracted from said illuminated surface; and
processing means for analysing said diffracted light for presence of a diffraction image representative of binding of one or more analytes with their analyte-specific receptors and identifying from said diffraction image one or more analytes present in said medium. - View Dependent Claims (35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 65)
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55-64. -64. (canceled)
Specification