Pichia pastoris formate dehydrogenase and uses therefor
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Abstract
This invention relates to a recombinant Pichia pastoris formate dehydrogenase (FDH) enzyme that catalyzes the oxidation of formate to carbon dioxide and the simultaneous reduction of nicotinamide adenine dinucleotide (NAD+) to its reduced form (NADH). Also related are isolated nucleic acids encoding P. pastoris FDH polypeptides, and fragments and variants thereof, as well as vectors and host cells comprising these nucleic acids. Further related are isolated, recombinant P. pastoris FDH polypeptides, and fragments and variants thereof, and antibodies that specifically bind to P. pastoris FDH polypeptides, fragments, or variants. The invention also relates to methods of obtaining isolated P. pastoris FDH nucleic acids, polypeptides, and antibodies, and methods of using P. pastoris FDH in various reactions for industrial or pharmaceutical applications.
35 Citations
37 Claims
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- 15. A recombinant polypeptide comprising amino acid sequence SEQ ID NO:
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16. A recombinant polypeptide comprising the NAD-binding domain of amino acid sequence SEQ ID NO:
- 5, wherein said recombinant polypeptide has formate dehydrogenase activity, and wherein the NAD-binding domain of amino acid sequence SEQ ID NO;
5 is amino acids 117-309 of SEQ ID NO;
5. - View Dependent Claims (26, 27, 29, 32, 35)
- 5, wherein said recombinant polypeptide has formate dehydrogenase activity, and wherein the NAD-binding domain of amino acid sequence SEQ ID NO;
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17. A recombinant polypeptide comprising the catalytic domain of amino acid sequence SEQ ID NO:
- 5, wherein said recombinant polypeptide has formate dehydrogenase activity, and wherein the catalytic domain of amino acid sequence SEQ ID NO;
5 is amino acids 16-115 of SEQ ID NO;
5. - View Dependent Claims (30, 33, 36)
- 5, wherein said recombinant polypeptide has formate dehydrogenase activity, and wherein the catalytic domain of amino acid sequence SEQ ID NO;
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19. A recombinant polypeptide that is a formate dehydrogenase encoded by the nucleotide sequence contained in the plasmid in the ATCC deposit designated PTA-3691.
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Specification