Donor yeast strain for transfer of genetic material

• US 20060105361A1
• Filed: 08/24/2005
• Published: 05/18/2006
• Est. Priority Date: 08/24/2004
• Status: Abandoned Application

• Current Assignee: Trustees Of Columbia University In The City Of New York (Columbia University)
• Original Assignee: Trustees Of Columbia University In The City Of New York (Columbia University)
• Inventors: Reid, Robert J.D., Rothstein, Rodney J., Sunjevaric, Ivana, Ciccone, Samantha, Wagner, Marisa
• Application Number:
  US11/211,359
• Publication Number:
  US 20060105361A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12N 15/81 for yeasts

Abstract

The invention provides a universal yeast donor strain that contains a conditional centromere and a URA3 allele on every chromosome. This strain was constructed in four rounds of crosses of individual conditional chromosome strains using a novel tetrad-based screen to identify segregants in which all marked chromosomes were contained in the same spore. The invention also provides an improved high efficiency method to transfer extrachromosomal genetic material such as plasmid DNA into any Saccharomyces strain for use with the current gene disruption libraries. The method of transfer is mating-based method which uses a kar1 plasmid donor strain that can initiate mating but cannot form a diploid and allows plasmid transfer (plasmoduction) between nuclei in the heterokaryon. kar1 matings have been used to transfer YACs between yeast strains, but previous methods required specialized genetic backgrounds in the recipient strains and suffered from high rates of spurious chromosome transfer (Hugerat, Y., et al. 1994. Genomics 22:108). Plasmoduction with the universal donor strain only requires that the recipient strain be ura3, GAL+ and have another marker available for selection of the transferred plasmid. Counterselection against every donor chromosome also limits the amount of spurious allele transfer. The universal donor strain and the method of the invention are used to screen the yeast gene disruption library with plasmid-based dominant negative alleles of various genes.

Claims

1. A yeast cell comprising chromosomes I through XVI, wherein at least two chromosomes comprise a nucleic acid sequence encoding a counterselectable marker linked to a centromere.

3. The yeast cell of claim 1 or 2, wherein the nucleic acid sequence further comprises a promoter capable of promoting transcription into the centromere of the chromosome, wherein the promoter is inducible or repressible.

4. The yeast cell of claim 3, wherein the counterselectable marker comprises a URA3 gene and the promoter comprises a GAL1 promoter.

5. The yeast cell of claim 1 or 2, wherein the yeast cell is of the genus Saccharomyces.

6. The yeast cell of claim 3, wherein the yeast cell comprises an extrachromosomal nucleic acid comprising a dominant selectable marker, and wherein the yeast genome lacks the selectable marker.

7. The yeast cell of claim 6, wherein lack of the selectable marker creates a metabolic deficiency or drug sensitivity.

8. The yeast cell of claim 7, wherein the selectable marker comprises LEU2, LYS5, TRP1, HIS3 prototrophic gene, the heterologous drug resistance marker KanMX or any combination thereof.

9. The yeast cell of claim 8, further comprising a mutation in the karyogamy pathway which permits yeast mating while preventing nuclear fusion.

10. A method for transferring extrachromosomal genetic material into a recipient yeast cell, the method comprising:

a) mating a recipient yeast cell to the donor yeast cell of claim 1 or 2, wherein at least one of the cells comprises a mutation in the karyogamy pathway,

b) selecting against the donor cell via the counterselectable marker, and

c) selecting for recipient cells which have acquired the extrachromosomal genetic material.

11. A method for transferring extrachromosomal genetic material into a recipient yeast cell, the method comprising:

a) mating a recipient cell to a donor yeast cell of claim 1 or 2, wherein the donor cell comprises a mutation in the karyogamy pathway,

b) selecting against the donor cell,

c) selecting for recipient cells which have acquired the extrachromosomal genetic material.

12. The method of claim 10 or claim 11, wherein the extrachromosomal genetic material comprises comprises a plasmid, an episomal nucleic acid, a mitochondrial nucleic acid or a virus.

13. The method of claim 10 or 11, wherein the steps are automated.

14. A method for transferring extrachromosomal material into a recipient yeast cell comprising the steps of:

a) mating a recipient cell to the donor yeast cell of claim 1 or 2, wherein at least one of the cells comprises a mutation in the karyogamy pathway,

b) selecting against the donor cell,

c) screening for recipient cells which have acquired the extrachromosomal material.

15. A method for transferring extrachromosomal material into a recipient yeast cell, the method comprising:

a) mating a recipient cell to a donor yeast cell of claim 1 or 2, wherein the donor cell comprises a mutation in the karyogamy pathway,

b) selecting against the donor cell,

c) screening for recipient cells which have acquired the extrachromosomal material.

16. The method of claim 14 or 15, wherein the extrachromosomal material comprises cytoplasm, a protein, a prion, an organelle or any combination thereof.

18. A method for identifying a nucleic acid sequence that exhibits synthetic dosage lethality in a yeast cell, the method comprising:

a) mating a recipient yeast cell to a donor yeast cell of claim 1 or 2, wherein at least one of the donor and recipient cells comprises a mutation in the karyogamy pathway,

b) selecting against the donor cell,

c) selecting for recipient cells which have acquired genetic material transferred from the donor cell,

d) identifying recipient cells which fail to grow due to failure to acquire the genetic material transferred from the donor cell.

19. The method of claim 18, wherein the donor cell expresses a human homologue of a gene associated with a proliferative disease.

20. The method of claim 19, wherein the proliferative disease comprises cancer.

21. The method of claim 18, wherein the recipient yeast cell comprises a cell from a yeast gene knockout library.

22. A method for transferring a yeast chromosome of interest from a donor yeast cell into a recipient yeast cell, the method comprising:

a) mating a recipient yeast cell to the donor yeast cell of claim 1, wherein at least one of the cells comprises a mutation in the karyogamy pathway,

b) selecting against the donor cell via the counterselectable marker, and

c) selecting for recipient cells which have acquired the yeast chromosome of interest, wherein the yeast chromosome of interest does not contain the counterselectable marker.

2. A yeast cell comprising chromosomes I through XVI, wherein each chromosome comprises a nucleic acid sequence encoding a counterselectable marker linked to a centromere.

17. A method for making the yeast cell of claim 2, the method comprising:

a) providing a diploid yeast cell that has one copy of chromosomes I through XVI marked with a counterselectable nucleic acid linked to the centromere, wherein the counterselectable nucleic acid comprises URA3,

b) sporulating the diploid,

c) plating the sporulated diploid to generate an original colony of adjacent haploid cells derived from the spores in an single ascus,

d) identifying an original colony which comprises spores that are uracil auxotrophs resistant to 5-FOA via growth on 5-FOA containing medium,

e) isolating uracil prototrophs spores that are adjacent to the uracil auxotrophs spores, and

f) performing a diagnostic PCR to confirm that all sixteen chromosomes in the uracil prototroph have the nucleic acid sequence which comprises URA3.