Immobilization support, process for producing the same, electrode, process for producing the same, electrode reaction utilizing apparatus and process for producing the same

• US 20060105418A1
• Filed: 05/21/2004
• Published: 05/18/2006
• Est. Priority Date: 06/05/2003
• Status: Active Grant

• Current Assignee: Murata Manufacturing Co Limited
• Original Assignee: Sony Corporation (Sony Group Corp.)
• Inventors: Sato, Atsushi, Kano, Kenji, Ikeda, Tokuji
• Granted Patent: US 7,520,970 B2
• Time in Patent Office: Days
• US Class Current: 435/25
• CPC Class Codes: C12Q 1/003 Functionalisation
• CPC Class Codes: H01M 4/86 Inert electrodes with catalytic activity, e.g. for fuel cells
• CPC Class Codes: H01M 8/16 Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
• CPC Class Codes: Y02E 60/50 Fuel cells
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

An immobilization carrier containing an electron acceptor compound is used in addition to glutaraldehyde and poly-L-lysine to immobilize an enzyme and an electron acceptor compound simultaneously to an electrode. For example, here are used diaphorase as the enzyme and 2-amino-3-carboxy-1,4-naphthoquinone (ACNQ) as the electron acceptor compound.

Claims

1. An immobilization carrier comprising glutaraldehyde and poly-L-lysine.

2. The immobilization carrier according to claim 1 further comprising an electron acceptor compound.

3. The immobilization carrier according to claim 2 wherein the electron acceptor compound has amino groups.

4. An immobilization carrier comprising a structure in which glutaraldehyde and poly-L-lysine are coupled by cross-linking.

5. An immobilization carrier comprising a first compound having at least two groups capable of polymerizing with a compound having amino groups or imino groups, and a second compound having amino groups or imino groups.

6. An immobilization carrier comprising a structure in which a first compound having at least two groups capable of polymerizing with a compound having amino groups or imino groups and a second compound having amino groups or imino groups are coupled by cross-linking.

7. An immobilization carrier comprising a third compound having at least two groups capable of polymerizing with a compound having carboxyl groups and a fourth compound having carboxyl groups.

8. An immobilization carrier comprising a structure in which a third compound having at least two groups capable of polymerizing with a compound having carboxyl groups and a fourth compound having carboxyl groups are coupled by cross-linking.

9. An electrode comprising an immobilization carrier containing glutaraldehyde and poly-L-lysine, and an enzyme.

10. The electrode according to claim 9 further comprising a coenzyme.

11. An electrode comprising an immobilization carrier which contains a first compound having at least two groups capable of polymerizing with amino groups or imino groups, and a second compound containing amino groups or imino groups;

and an enzyme.

12. An electrode comprising an immobilization carrier having a structure in which a first compound having at least two groups capable of polymerizing with amino groups or imino groups and a second compound containing amino groups or imino groups are coupled by cross-linking;

and an enzyme.

13. An electrode comprising an immobilization carrier containing a third compound having at least two groups capable of polymerizing with a compound having carboxyl groups, and a fourth compound having carboxyl groups;

and an enzyme.

14. An electrode comprising an immobilization carrier containing a third compound having at least two groups capable of polymerizing with a compound having carboxyl groups, and a fourth compound having carboxyl groups;

and an enzyme.

15. An electrode reaction-utilizing device using an electrode containing an immobilization carrier and an enzyme, characterized in that the immobilization carrier comprises a first compound having at least two groups capable of polymerizing with a compound having amino groups or imino groups, and a second compound having amino groups or imino groups.

16. An electrode reaction-utilizing device using an electrode containing an immobilization carrier and an enzyme, characterized in that the immobilization carrier has a structure in which a first compound having at least two groups capable of polymerizing with a compound having amino groups or imino groups and a second compound having amino groups or imino groups are coupled by cross-linking.

17. An electrode reaction-utilizing device using an electrode containing an immobilization carrier and an enzyme, characterized in that the immobilization carrier contains a third compound having at least two groups capable of polymerizing with a compound having carboxyl groups and a fourth compound having carboxyl groups.

18. An electrode reaction-utilizing device using an electrode containing an immobilization carrier and an enzyme, characterized in that the immobilization carrier has a structure in which a third compound having at least two groups capable of polymerizing with a compound having carboxyl groups and a fourth compound having carboxyl groups are coupled by cross-linking.

19. A method of manufacturing an immobilization carrier, comprising:

polymerizing a first compound having at least two groups capable of polymerizing with a compound having amino groups or imino groups and a second compound having amino groups or imino groups.

20. A method of manufacturing an immobilization carrier, comprising:

polymerizing third compound having at least two groups capable of polymerizing with a compound having amino groups or imino groups and a fourth compound having amino groups or imino groups.

21. A method of manufacturing an electrode, comprising:

immobilizing an enzyme to an immobilization carrier containing a first compound having at least two groups capable of polymerizing with amino groups or imino groups and a second compound having amino groups or imino groups.

22. A method of manufacturing an electrode, comprising:

immobilizing an enzyme to an immobilization carrier containing a third compound having at least two groups capable of polymerizing with a compound having carboxyl groups and a fourth compound having carboxyl groups.

23. A method of manufacturing an electrode, comprising:

immobilizing an enzyme to an immobilization carrier obtained by polymerization of a first compound having at least two groups capable of polymerizing with a compound having amino groups or imino groups and a second compound having amino groups or imino groups.

24. A method of manufacturing an electrode, comprising:

immobilizing an enzyme to an immobilization carrier obtained by polymerization of a third compound having at least two groups capable of polymerizing with a compound having carboxyl groups and a fourth compound having carboxyl groups.

25. A method of manufacturing an electrode reaction-utilizing device using an electrode containing an immobilization carrier and an enzyme, comprising the step of:

manufacturing the electrode by immobilizing the enzyme to the immobilization carrier containing a first compound having at least two groups capable of polymerizing with a compound having amino groups or imino groups and a second compound having amino groups or imino groups.

26. A method of manufacturing an electrode reaction-utilizing device using an electrode containing an immobilization carrier and an enzyme, comprising the step of:

manufacturing the electrode by immobilizing the enzyme to the immobilization carrier containing a third compound having at least two groups capable of polymerizing with a compound having carboxyl groups with a fourth compound having carboxyl groups.

27. A method of manufacturing an electrode reaction-utilizing device using an electrode containing an immobilization carrier and an enzyme, comprising the step of:

manufacturing the electrode by immobilizing the enzyme to the immobilization carrier obtained by polymerization of a first compound having at least two groups capable of polymerizing with a compound having amino groups or imino groups and a second compound having amino groups or imino groups.

28. A method of manufacturing an electrode reaction-utilizing device using an electrode containing an immobilization carrier and an enzyme, comprising the step of:

manufacturing the electrode by immobilizing the enzyme to the immobilization carrier obtained by polymerization of a third compound having at least two groups capable of polymerizing with a compound having carboxyl groups and a fourth compound having carboxyl groups.