Nanopore analysis system
First Claim
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1. A nanopore analysis system, comprising:
- an automated nanopore system including;
a nanopore chamber and a nanopore structure disposed within, the nanopore structure having a nanopore aperture; and
a vacuum system interfaced with the nanopore chamber, wherein the vacuum system is configured to remove a gas bubble within a liquid disposed in the nanopore chamber without removing the nanopore structure from the nanopore chamber.
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Abstract
Nanopore analysis systems, methods of preparing nanopore analysis systems, and methods of automating the analysis of samples using nanopore analysis systems, are provided.
132 Citations
14 Claims
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1. A nanopore analysis system, comprising:
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an automated nanopore system including;
a nanopore chamber and a nanopore structure disposed within, the nanopore structure having a nanopore aperture; and
a vacuum system interfaced with the nanopore chamber, wherein the vacuum system is configured to remove a gas bubble within a liquid disposed in the nanopore chamber without removing the nanopore structure from the nanopore chamber. - View Dependent Claims (2)
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3. A nanopore analysis system, comprising:
an automated nanopore system including a nanopore chamber and a nanopore structure disposed within the nanopore chamber, wherein the nanopore structure includes a nanopore aperture, wherein the automated nanopore system is configured to introduce a plurality of liquid samples into the nanopore chamber in an automated manner, wherein the automated nanopore system is configured to remove the plurality of liquid samples from the nanopore chamber in an automated manner, wherein the automated nanopore system is configured in a manner whereby each sample can be introduced and removed from the nanopore chamber without removing the nanopore structure from the nanopore chamber, and wherein the liquid sample is selected from a sample liquid and a buffer liquid. - View Dependent Claims (4, 5)
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6. A method of preparing a nanopore analysis system, comprising:
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providing an automated nanopore system including a nanopore chamber having a nanopore structure, wherein the nanopore structure includes a nanopore aperture;
disposing a first volume of a liquid in the nanopore chamber, wherein at least one gas bubble is formed within the liquid in the nanopore chamber, and wherein the gas bubble is substantially blocking the nanopore aperture;
evacuating the liquid from the nanopore chamber to remove the gas bubble; and
flowing a second volume of a liquid into the nanopore chamber, wherein the nanopore aperture is not substantially blocked by gas bubbles. - View Dependent Claims (7, 8, 9)
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10. A method of preparing a nanopore analysis system, comprising:
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providing a nanopore system including a nanopore chamber and a nanopore structure having a nanopore aperture, wherein the nanopore chamber has first side and a second side, each being disposed on opposite sides of the nanopore structure, wherein the nanopore aperture fluidicly connects the first side with the second side;
disposing a liquid into the first side and the second side of the nanopore chamber, wherein at least one gas bubble is formed within the liquid, and wherein the gas bubble is substantially blocking the nanopore aperture; and
evacuating the liquid from the first side of the nanopore chamber, wherein the evacuation causes the liquid from the second side to flow through the nanopore aperture to the first side, and wherein the flow of liquid from the second side to the first side removes the gas bubble blocking the nanopore aperture. - View Dependent Claims (11, 12, 13)
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14. A method of automating the analysis of samples using a nanopore analysis system, comprising:
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providing a nanopore system including a nanopore chamber and a nanopore structure having a nanopore aperture;
disposing a first volume of a buffer liquid in the nanopore chamber, wherein at least one gas bubble is formed within the buffer fluid in the nanopore chamber, and wherein the gas bubble is substantially blocking the nanopore aperture;
evacuating the buffer liquid from the nanopore chamber to remove the gas bubble;
flowing a first sample into the nanopore chamber, wherein the first sample includes a first polymer, wherein the nanopore aperture is not substantially blocked by gas bubbles;
analyzing the first polymer using the nanopore system;
removing the sample from the nanopore chamber;
disposing a second volume of the buffer liquid in the nanopore chamber;
removing the second volume of the buffer liquid from the nanopore chamber;
flowing a second sample into the nanopore chamber, wherein the second sample includes a second polymer, wherein the nanopore aperture is not substantially blocked by gas bubbles; and
analyzing the second polymer using the nanopore system, wherein the nanopore structure is not removed from the nanopore chamber to remove the gas bubble.
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Specification