×

Amplification and separation of nucleic acid sequences using strand displacement amplification and bioelectronic microchip technology

  • US 20060110754A1
  • Filed: 10/14/2005
  • Published: 05/25/2006
  • Est. Priority Date: 04/12/1999
  • Status: Abandoned Application
First Claim
Patent Images

1. A method for the amplification, multiplex assaying, and detection target nucleic acids of interest using a bioelectronic microchip, comprising the steps of:

  • a) introducing at least one of the target nucleic acids onto a bioelectronic microchip having a plurality of electronically addressable capture sites;

    b) amplifying said target nucleic acids to form amplicons of said target nucleic acids;

    c) providing for said amplicons to contact capture probes located on capture sites by a process wherein said process comprises either (1) biasing any of said plurality of electronically addressable capture sites with an electronic bias sufficient to attract said amplicons to said biased capture sites, or (2) hybridizing said amplicons with said capture probes without electronic biasing;

    d) capturing said amplicons onto specified electronically addressable capture sites of (c) by a capture probe that has specificity for said amplicons; and

    e) detecting the presence of said amplicons;

    wherein amplification of the target nucleic acid is by strand displacement amplification.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×