×

Detection of nucleic acid variation by cleavage-amplification (CleavAmp) method

  • US 20060110765A1
  • Filed: 11/18/2005
  • Published: 05/25/2006
  • Est. Priority Date: 11/23/2004
  • Status: Abandoned Application
First Claim
Patent Images

1. A method for detecting a polymorphism in a polynucleotide, comprising:

  • (a) annealing a probe to a region of a polynucleotide suspected of containing a polymorphism to form a complex, wherein the probe comprises a non-extendable 3′

    end and is not complementary to the polymorphism;

    (b) contacting the complex with an enzyme or chemical that cleaves the probe and the polynucleotide at a region of mismatch between the probe and the polynucleotide to produce a probe with an extendible 3′

    end;

    (c) adding an artificial template, wherein the cleaved probe acts as a primer for amplifying the artificial template; and

    (d) amplifying the artificial template, wherein the presence of an amplified product indicates the presence of the polymorphism.

View all claims
  • 0 Assignments
Timeline View
Assignment View
    ×
    ×