Novel exothermic material, method and device for lysing cells using the exothermic material

• US 20060115873A1
• Filed: 11/30/2005
• Published: 06/01/2006
• Est. Priority Date: 12/01/2004
• Status: Abandoned Application

• Current Assignee: Samsung Electronics Co. Ltd.
• Original Assignee: Samsung Electronics Co. Ltd.
• Inventors: Park, Jong-myeon, Peak, Sang-hyun, Lee, Jung-nam, Chung, Jong-Suk
• Application Number:
  US11/289,857
• Publication Number:
  US 20060115873A1
• Time in Patent Office: Days
• US Class Current: 435/41
• CPC Class Codes: B01L 2300/1877 using chemical reactions
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/508 rigid containers not provided for above
• CPC Class Codes: B01L 7/00 Heating or cooling apparatus autoclaves B01J3/04; Heat insulating devices
• CPC Class Codes: C12M 47/06 Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material lysis of microorganisms C12N1/06; extracting or separating nucleic acids from biological samples C12N15/1003

Abstract

Provided are an exothermic material, and a method and device for lysing cells using the exothermic material. The exothermic material is a solid material containing CaO, 5-7% by weight of MgCl₂ or MgSO₄, and 5-7% by weight of NaOH, with respect to the total weight of CaO, and generates heat upon the addition of sulfuric acid.

Claims

1. An exothermic material which is a solid material containing CaO, 5-7% by weight of MgCl₂ or MgSO₄ and 5-7% by weight of NaOH, with respect to the total weight of CaO, and generates heat upon the addition of sulfuric acid.

2. A method of lysing cells using heat generated by an electrochemical reaction, the method comprising:

introducing cells into a container; and

transferring to the container the heat generated upon the addition of a 0.1 to 5 M sulfuric acid solution to a solid material containing CaO, 5-7% by weight of MgCl₂ or MgSO₄, and 5-7% by weight of NaOH, with respect to the total weight of CaO.

3. The method according to claim 2, wherein the container is a microchannel or a cavity in a microfluidic device.

4. A device for lysing cells using heat generated by an electrochemical reaction, the device comprising:

a container to hold cells; and

a compartment adjacent to the container, the compartment containing a solid exothermic material containing CaO, 5-7% by weight of MgCl₂ or MgSO₄, and 5-7% by weight of NaOH, with respect to the total weight of CaO, and having an inlet for introducing sulfuric acid.

5. The device according to claim 5, wherein the container and the compartment are microchannels or microchambers.