Polymer grafting by polysaccharide synthases

• US 20060116348A1
• Filed: 07/11/2005
• Published: 06/01/2006
• Est. Priority Date: 11/11/1998
• Status: Active Grant

• Current Assignee: Paul L. Deangelis
• Original Assignee: Paul L. Deangelis
• Inventors: DeAngelis, Paul L.
• Granted Patent: US 7,575,904 B2
• Time in Patent Office: Days
• US Class Current: 514/54
• CPC Class Codes: A61K 31/728 Hyaluronic acid
• CPC Class Codes: A61K 31/737 Sulfated polysaccharides, e.g. chondroitin sulfate, dermatan sulfate A61K31/727 takes precedence
• CPC Class Codes: A61K 47/36 Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
• CPC Class Codes: A61K 9/006 Oral mucosa, e.g. mucoadhesive forms, sublingual droplets; Buccal patches or films; Buccal sprays
• CPC Class Codes: A61L 24/08 Polysaccharides A61L24/043 takes precedence
• CPC Class Codes: A61L 29/085 Macromolecular materials
• CPC Class Codes: C08L 5/08 Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
• CPC Class Codes: C12N 9/1048 Glycosyltransferases (2.4)
• CPC Class Codes: C12N 9/1051 Hexosyltransferases (2.4.1)
• CPC Class Codes: C12P 19/26 Preparation of nitrogen-containing carbohydrates
• CPC Class Codes: C12P 19/28 N-glycosides
• CPC Class Codes: Y10S 977/898 Enzymatic

Abstract

The present invention relates to methodology for polymer grafting by a polysaccharide synthase and, more particularly, polymer grafting using the hyaluronate synthase from Pasteurella multocida. The present invention also relates to coatings for biomaterials wherein the coatings provide protective properties to the biomaterial and/or act as a bioadhesive. Such coatings could be applied to electrical devices, sensors, catheters and any device which may be contemplated for use within a mammal. The present invention further relates to drug delivery matrices which are biocompatible and may comprise combinations of a biomaterial or a bioadhesive and a medicament or a medicament-containing liposome. The biomaterial and/or bioadhesive is a hyaluronic acid polymer produced by a hyaluronate synthase from Pasteurella multocida. The present invention also relates to the creation of chimeric molecules containing hyaluronic acid or hyaluronic acid-like chains attached to various compounds and especially carbohydrates or hydroxyl containing substances. The present invention also relates to a chondroitan synthase from Pasteurella multocida which is capable of producing polysaccharide polymers on an acceptor or primer molecule.

Claims

1. A method for producing a polysaccharide polymer on a substrate, comprising the steps of:

providing a substrate having an immobilized polysaccharide primer thereon to provide a primed substrate;

combining the primed substrate with a PmHAS-D enzyme within a reaction medium, wherein the reaction medium contains at least one sugar precursor selected from the group consisting of UDP-GlcA and UDP-GlcNAc; and

reacting the PmHAS-D enzyme with the primed substrate to produce a substrate having a polysaccharide polymer coated thereon.

2. The method of claim 1, wherein the polysaccharide polymer is hyaluronic acid.

3. The method of claim 1, wherein the polysaccharide polymer is chondroitin.

4. A substrate coated with a polysaccharide polymer manufactured according to the process of claim 1.

5. The substrate of claim 4, wherein the polysaccharide polymer is hyaluronic acid.

6. The substrate of claim 4, wherein the polysaccharide polymer is chondroitin.

7. A method for producing a polysaccharide bioadhesive sealant, comprising the steps of:

combining an enzyme selected from the group consisting of PmHAS-D and PmCS with a reaction medium, wherein the reaction medium contains at least one sugar precursor selected from the group consisting of UDP-GlcA, UDP-GlcNAc or UDP-GalNAc; and

allowing the enzyme to react with the reaction medium, wherein the enzyme produces a polysaccharide polymer capable of use as a bioadhesive sealant by successively combining the at least one sugar precursor into a polymer chain.

8. A polysaccharide bioadhesive sealant manufactured according to the process of claim 7.

9. A method for producing a polysaccharide biomaterial containing a medicament delivery assembly, comprising the steps of:

preparing a polysaccharide polymer from an enzyme selected from the group consisting of PmHAS or PmCS, wherein the polysaccharide polymer is capable of acting as a bioadhesive;

providing at least one medicament delivery assembly containing one or more medicaments entrapped therein; and

mixing the prepared polysaccharide bioadhesive with the at least one medicament delivery assembly, wherein the prepared polysaccharide bioadhesive entraps the at least one medicament delivery assembly to produce a polysaccharide biomaterial containing a medicament delivery system.

10. The method of claim 9 wherein the polysaccharide biomaterial containing a medicament delivery assembly is deliverable within a wound, ulcer, injury or surgical site.

11. A polysaccharide biomaterial containing a medicament delivery assembly manufactured according to the process of claim 9.

12. A method for inducing coagulation of a medicament delivery assembly-containing polysaccharide biomaterial composition at a site of injury, wound, or surgery in a mammal, thereby resulting in sealing and healing of the injury, wound or surgical site, comprising the steps of:

preparing a medicament delivery assembly-containing polysaccharide bioadhesive composition from an enzyme selected from the group consisting of PmHAS or PmCS; and

administering the medicament delivery assembly-containing polysaccharide bioadhesive composition to a mammal, wherein the coagulation of the medicament delivery assembly-containing polysaccharide bioadhesive composition forms a medium for entrapping the medicament delivery assembly at the site and embeds the medicament delivery assembly in the polysaccharide biomaterial composition.

13. A method for hemostatically sealing and healing a wound or surgical incision and delivering to the wound or incision medicaments, comprising the steps of:

preparing a polysaccharide biomaterial composition having a medicament containing delivery assembly, wherein the polysaccharide bioadhesive composition is prepared from an enzyme selected from the group consisting of PmHAS and PmCS; and

administering the composition to the surface of a wound or incision, wherein coagulation of the composition forms a medium for entrapping the medicament delivery assembly at the site, embeds the medicament delivery assembly in the composition, and allows for a sustained release of the medicament delivery assembly contents to the wound or incision.

14. A biomaterial composition, comprising:

an effective amount of a polysaccharide polymer produced from an enzyme selected from the group consisting of PmHAS-D and PmCS; and

at least one medicament delivery assembly containing at least one medicament, wherein the at least one medicament delivery assembly is embedded in the polysaccharide polymer so as to localize the at least one medicament within the polysaccharide polymer bioadhesive composition.