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Polymer grafting by polysaccharide synthases

  • US 20060116348A1
  • Filed: 07/11/2005
  • Published: 06/01/2006
  • Est. Priority Date: 11/11/1998
  • Status: Active Grant
First Claim
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1. A method for producing a polysaccharide polymer on a substrate, comprising the steps of:

  • providing a substrate having an immobilized polysaccharide primer thereon to provide a primed substrate;

    combining the primed substrate with a PmHAS-D enzyme within a reaction medium, wherein the reaction medium contains at least one sugar precursor selected from the group consisting of UDP-GlcA and UDP-GlcNAc; and

    reacting the PmHAS-D enzyme with the primed substrate to produce a substrate having a polysaccharide polymer coated thereon.

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