Fad4, Fad5, Fad5-2, and Fad6, novel fatty acid desaturase family members and uses thereof
First Claim
Patent Images
1. An isolated nucleic acid molecule selected from the group consisting of a) an isolated nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO:
- 7;
b) an isolated nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO;
8;
c) an isolated nucleic acid molecule comprising a nucleotide sequence which is at least 95% identical to the nucleotide sequence set forth in SEQ ID NO;
7 and wherein the nucleic acid molecule encodes a Δ
6 fatty acid desaturase molecule capable of converting linoleic acid (18;
2) to gamma-linolenic acid;
d) an isolated nucleic acid molecule comprising a nucleotide sequence which is at least 95% identical to the nucleotide sequence set forth in SEQ ID NO;
7 and wherein the nucleic acid molecule encodes a Δ
6 fatty acid desaturase molecule capable of converting linolenic acid (18;
3) to stearidonic acid;
e) an isolated nucleic acid molecule which encodes a polypeptide comprising an amino acid sequence which is at least 95% identical to the amino acid sequence set forth in SEQ ID NO;
8 and wherein the polypeptide is a Δ
6 fatty acid desaturase molecule capable of converting linoleic acid (18;
2) to gamma-linolenic acid;
f) an isolated nucleic acid molecule which encodes a polypeptide comprising an amino acid sequence which is at least 95% identical to the amino acid sequence set forth in SEQ ID NO;
8 and wherein the polypeptide is a Δ
6 fatty acid desaturase molecule capable of converting linolenic acid (18;
3) to stearidonic acid;
g) an isolated nucleic acid molecule encoding a Δ
6 fatty acid desaturase molecule capable of converting linoleic acid (18;
2) to gamma-linolenic acid, wherein the desaturase molecule comprises an amino acid sequence which is at least 70% identical to the amino acid sequence set forth in SEQ ID NO;
8 and further comprises a heme-binding motif and a histidine-rich motif;
h) an isolated nucleic acid molecule encoding a Δ
6 fatty acid desaturase molecule capable of converting linolenic acid (18;
3) to stearidonic acid, wherein the desaturase molecule comprises an amino acid sequence which is at least 70% identical to the amino acid sequence set forth in SEQ ID NO;
8 and further comprises a heme-binding motif and a histidine-rich motif; and
i) an isolated nucleic acid molecule comprising a nucleotide sequence of at least 12 contiguous nucleotides of SEQ ID NO;
7.
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Abstract
The invention provides isolated nucleic acid molecules which encode novel fatty acid desaturase family members. The invention also provides recombinant expression vectors containing desaturase nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., DHA.
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Citations
14 Claims
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1. An isolated nucleic acid molecule selected from the group consisting of
a) an isolated nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO: - 7;
b) an isolated nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO;
8;
c) an isolated nucleic acid molecule comprising a nucleotide sequence which is at least 95% identical to the nucleotide sequence set forth in SEQ ID NO;
7 and wherein the nucleic acid molecule encodes a Δ
6 fatty acid desaturase molecule capable of converting linoleic acid (18;
2) to gamma-linolenic acid;
d) an isolated nucleic acid molecule comprising a nucleotide sequence which is at least 95% identical to the nucleotide sequence set forth in SEQ ID NO;
7 and wherein the nucleic acid molecule encodes a Δ
6 fatty acid desaturase molecule capable of converting linolenic acid (18;
3) to stearidonic acid;
e) an isolated nucleic acid molecule which encodes a polypeptide comprising an amino acid sequence which is at least 95% identical to the amino acid sequence set forth in SEQ ID NO;
8 and wherein the polypeptide is a Δ
6 fatty acid desaturase molecule capable of converting linoleic acid (18;
2) to gamma-linolenic acid;
f) an isolated nucleic acid molecule which encodes a polypeptide comprising an amino acid sequence which is at least 95% identical to the amino acid sequence set forth in SEQ ID NO;
8 and wherein the polypeptide is a Δ
6 fatty acid desaturase molecule capable of converting linolenic acid (18;
3) to stearidonic acid;
g) an isolated nucleic acid molecule encoding a Δ
6 fatty acid desaturase molecule capable of converting linoleic acid (18;
2) to gamma-linolenic acid, wherein the desaturase molecule comprises an amino acid sequence which is at least 70% identical to the amino acid sequence set forth in SEQ ID NO;
8 and further comprises a heme-binding motif and a histidine-rich motif;
h) an isolated nucleic acid molecule encoding a Δ
6 fatty acid desaturase molecule capable of converting linolenic acid (18;
3) to stearidonic acid, wherein the desaturase molecule comprises an amino acid sequence which is at least 70% identical to the amino acid sequence set forth in SEQ ID NO;
8 and further comprises a heme-binding motif and a histidine-rich motif; and
i) an isolated nucleic acid molecule comprising a nucleotide sequence of at least 12 contiguous nucleotides of SEQ ID NO;
7. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
- 7;
Specification