Protein kinases
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention relates to novel proteins, termed INSP081, INSP082, and INSP091, herein identified as members of the Germinal Center Kinase (GCK) subfamily of the STE20 family of protein kinases, preferably as NCK-interacting kinases (NIKs) and more preferably as NIK-like embryo specific kinases (NESKs), and to the use of these and nucleic acid sequences from the encoding genes in the diagnosis, prevention, and treatment of disease.
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Citations
62 Claims
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1-54. -54. (canceled)
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55. A composition of matter:
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(a) comprising an amino acid sequence as recited in SEQ ID NO;
2, SEQ ID NO;
4, SEQ ID NO;
6, SEQ ID NO;
8, SEQ ID NO;
10, SEQ ID NO;
12, SEQ ID NO;
14, SEQ ID NO;
16, SEQ ID NO;
18, SEQ ID NO;
20, SEQ ID NO;
22, SEQ ID NO;
24, SEQ ID NO;
26, SEQ ID NO;
28, SEQ ID NO;
30, SEQ ID NO;
32, SEQ ID NO;
34, SEQ ID NO;
36, SEQ ID NO;
38, SEQ ID NO;
40, SEQ ID NO;
42, SEQ ID NO;
44, SEQ ID NO;
46, SEQ ID NO;
48, SEQ ID NO;
50, SEQ ID NO;
52, SEQ ID NO;
54, SEQ ID NO;
56, SEQ ID NO;
58, SEQ ID NO;
60, SEQ ID NO;
62, SEQ ID NO;
64, SEQ ID NO;
66, SEQ ID NO;
68, SEQ ID NO;
70, SEQ ID NO;
72, SEQ ID NO;
74, SEQ ID NO;
76, SEQ ID NO;
78, SEQ ID NO;
80, SEQ ID NO;
82, SEQ ID NO;
84, SEQ ID NO;
86, SEQ ID NO;
88, SEQ ID NO;
90, SEQ ID NO;
92, SEQ ID NO;
94, SEQ ID NO;
96, SEQ ID NO;
98, SEQ ID NO;
100, SEQ ID NO;
102, SEQ ID NO;
104, SEQ ID NO;
106, SEQ ID NO;
108, SEQ ID NO;
110, SEQ ID NO;
112, SEQ ID NO;
114, SEQ ID NO;
116, SEQ ID NO;
118, SEQ ID NO;
120, SEQ ID NO;
122, SEQ ID NO;
124, SEQ ID NO;
126, SEQ ID NO;
128, SEQ ID NO;
130, SEQ ID NO;
132, SEQ ID NO;
134, SEQ ID NO;
136, SEQ ID NO;
138, SEQ ID NO;
140, SEQ ID NO;
142, SEQ ID NO;
144, SEQ ID NO;
146, SEQ ID NO;
148, SEQ ID NO;
150, SEQ ID NO;
152, SEQ ID NO;
154, SEQ ID NO;
156 or SEQ ID NO;
158;
(b) consisting of an amino acid sequence as recited in SEQ ID NO;
2, SEQ ID NO;
4, SEQ ID NO;
6, SEQ ID NO;
8, SEQ ID NO;
10, SEQ ID NO;
12, SEQ ID NO;
14, SEQ ID NO;
16, SEQ ID NO;
18, SEQ ID NO;
20, SEQ ID NO;
22, SEQ ID NO;
24, SEQ ID NO;
26, SEQ ID NO;
28, SEQ ID NO;
30, SEQ ID NO;
32, SEQ ID NO;
34, SEQ ID NO;
36, SEQ ID NO;
38, SEQ ID NO;
40, SEQ ID NO;
42, SEQ ID NO;
44, SEQ ID NO;
46, SEQ ID NO;
48, SEQ ID NO;
50, SEQ ID NO;
52, SEQ ID NO;
54, SEQ ID NO;
56, SEQ ID NO;
58, SEQ ID NO;
60, SEQ ID NO;
62, SEQ ID NO;
64, SEQ ID NO;
66, SEQ ID NO;
68, SEQ ID NO;
70, SEQ ID NO;
72, SEQ ID NO;
74, SEQ ID NO;
76, SEQ ID NO;
78, SEQ ID NO;
80, SEQ ID NO;
82, SEQ ID NO;
84, SEQ ID NO;
86, SEQ ID NO;
88, SEQ ID NO;
90, SEQ ID NO;
92, SEQ ID NO;
94, SEQ ID NO;
96, SEQ ID NO;
98, SEQ ID NO;
100, SEQ ID NO;
102, SEQ ID NO;
104, SEQ ID NO;
106, SEQ ID NO;
108, SEQ ID NO;
110, SEQ ID NO;
112, SEQ ID NO;
114, SEQ ID NO;
116, SEQ ID NO;
118, SEQ ID NO;
120, SEQ ID NO;
122, SEQ ID NO;
124, SEQ ID NO;
126, SEQ ID NO;
128, SEQ ID NO;
130, SEQ ID NO;
132, SEQ ID NO;
134, SEQ ID NO;
136, SEQ ID NO;
138, SEQ ID NO;
140, SEQ ID NO;
142, SEQ ID NO;
144, SEQ ID NO;
146, SEQ ID NO;
148, SEQ ID NO;
150, SEQ ID NO;
152, SEQ ID NO;
154, SEQ ID NO;
156 or SEQ ID NO;
158;
(c) that is a fragment of the amino acid sequence of (a) or (b) and which is a member of the Germinal Center Kinase (GCK) subfamily of the STE20 family of protein kinases, a NIK-like kinase, a NIK-like embryo specific kinase (NESK), or having an antigenic determinant in common with the polypeptide of (a) or (b);
(d) that is a functional equivalent of (a), (b) or (c);
(e) comprising an isolated polypeptide that is a functional equivalent of (a), (b) or (c) and is homologous to the amino acid sequence as recited in SEQ ID NO;
2, SEQ ID NO;
4, SEQ ID NO;
6, SEQ ID NO;
8, SEQ ID NO;
10, SEQ ID NO;
12, SEQ ID NO;
14, SEQ ID NO;
16, SEQ ID NO;
18, SEQ ID NO;
20, SEQ ID NO;
22, SEQ ID NO;
24, SEQ ID NO;
26, SEQ ID NO;
28, SEQ ID NO;
30, SEQ ID NO;
32, SEQ ID NO;
34, SEQ ID NO;
36, SEQ ID NO;
38, SEQ ID NO;
40, SEQ ID NO;
42, SEQ ID NO;
44, SEQ ID NO;
46, SEQ ID NO;
48, SEQ ID NO;
50, SEQ ID NO;
52, SEQ ID NO;
54, SEQ ID NO;
56, SEQ ID NO;
58, SEQ ID NO;
60, SEQ ID NO;
62, SEQ ID NO;
64, SEQ ID NO;
66, SEQ ID NO;
68, SEQ ID NO;
70, SEQ ID NO;
72, SEQ ID NO;
74, SEQ ID NO;
76, SEQ ID NO;
78, SEQ ID NO;
80, SEQ ID NO;
82, SEQ ID NO;
84, SEQ ID NO;
86, SEQ ID NO;
88, SEQ ID NO;
90, SEQ ID NO;
92, SEQ ID NO;
94, SEQ ID NO;
96, SEQ ID NO;
98, SEQ ID NO;
100, SEQ ID NO;
102, SEQ ID NO;
104, SEQ ID NO;
106, SEQ ID NO;
108, SEQ ID NO;
110, SEQ ID NO;
112, SEQ ID NO;
114, SEQ ID NO;
116, SEQ ID NO;
118, SEQ ID NO;
120, SEQ ID NO;
122, SEQ ID NO;
124, SEQ ID NO;
126, SEQ ID NO;
128, SEQ ID NO;
130, SEQ ID NO;
132, SEQ ID NO;
134, SEQ ID NO;
136, SEQ ID NO;
138, SEQ ID NO;
140, SEQ ID NO;
142, SEQ ID NO;
144, SEQ ID NO;
146, SEQ ID NO;
148, SEQ ID NO;
150, SEQ ID NO;
152, SEQ ID NO;
154, SEQ ID NO;
156 or SEQ ID NO;
158 and is a member of the Germinal Center Kinase (GCK) subfamily of the STE20 family of protein kinases;
(f) comprising an isolated polypeptide which is a fragment or functional equivalent of a polypeptide as set forth in (a), (b), (c), (d), or (e), and that has greater than 80% sequence identity with the amino acid sequence recited in SEQ ID NO;
2, SEQ ID NO;
4, SEQ ID NO;
6, SEQ ID NO;
8, SEQ ID NO;
10, SEQ ID NO;
12, SEQ ID NO;
14, SEQ ID NO;
16, SEQ ID NO;
18, SEQ ID NO;
20, SEQ ID NO;
22, SEQ ID NO;
24, SEQ ID NO;
26, SEQ ID NO;
28, SEQ ID NO;
30, SEQ ID NO;
32, SEQ ID NO;
34, SEQ ID NO;
36, SEQ ID NO;
38, SEQ ID NO;
40, SEQ ID NO;
42, SEQ ID NO;
44, SEQ ID NO;
46, SEQ ID NO;
48, SEQ ID NO;
50, SEQ ID NO;
52, SEQ ID NO;
54, SEQ ID NO;
56, SEQ ID NO;
58, SEQ ID NO;
60, SEQ ID NO;
62, SEQ ID NO;
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66, SEQ ID NO;
68, SEQ ID NO;
70, SEQ ID NO;
72, SEQ ID NO;
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100, SEQ ID NO;
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128, SEQ ID NO;
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134, SEQ ID NO;
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140, SEQ ID NO;
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146, SEQ ID NO;
148, SEQ ID NO;
150, SEQ ID NO;
152, SEQ ID NO;
154, SEQ ID NO;
156, SEQ ID NO;
158 or with an active fragment thereof;
(g) which is a polypeptide fragment, or functional equivalent thereof, and has an antigenic determinant in common with a polypeptide which consists of 7 or more amino acid residues from the amino acid sequence recited in SEQ ID NO;
2, SEQ ID NO;
4, SEQ ID NO;
6, SEQ ID NO;
8, SEQ ID NO;
10, SEQ ID NO;
12, SEQ ID NO;
14, SEQ ID NO;
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20, SEQ ID NO;
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32, SEQ ID NO;
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36, SEQ ID NO;
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40, SEQ ID NO;
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44, SEQ ID NO;
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48, SEQ ID NO;
50, SEQ ID NO;
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54, SEQ ID NO;
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60, SEQ ID NO;
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150, SEQ ID NO;
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154, SEQ ID NO;
156 or SEQ ID NO;
158;
(h) comprising a purified nucleic acid molecule which encodes a polypeptide according to (a), (b), (c), (d), (e), (f), or (g);
(i) comprising a purified nucleic acid sequence as recited in SEQ ID NO;
1, SEQ ID NO;
3, SEQ ID NO;
5, SEQ ID NO;
7, SEQ ID NO;
9, SEQ ID NO;
11, SEQ ID NO;
13, SEQ ID NO;
15, SEQ ID NO;
17, SEQ ID NO;
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25, SEQ ID NO;
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45, SEQ ID NO;
47 SEQ ID NO;
49, SEQ ID NO;
5 1, SEQ ID NO;
53, SEQ ID NO;
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59, SEQ ID NO;
61, SEQ ID NO;
63, SEQ ID NO;
65, SEQ ID NO;
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71, SEQ ID SEQ ID NO;
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117, SEQ ID NO;
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121, SEQ ID NO;
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145, SEQ ID NO;
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149, SEQ ID NO;
151, SEQ ID NO;
153, SEQ ID NO;
155, SEQ ID NO;
157 or is a redundant equivalent or fragment thereof;
(j) consisting of a purified nucleic acid sequence as recited in SEQ ID NO;
1, SEQ ID NO;
3, SEQ ID NO;
5, SEQ ID NO;
7, SEQ ID NO;
9, SEQ ID NO;
11, SEQ ID NO;
13, SEQ ID NO;
15, SEQ ID NO;
17, SEQ ID NO;
19, SEQ ID NO;
21, SEQ ID NO;
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25, SEQ ID NO;
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47 SEQ ID NO;
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61, SEQ ID NO;
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69, SEQ ID NO;
71, SEQ ID SEQ ID NO;
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117, SEQ ID NO;
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121, SEQ ID NO;
123, SEQ ID NO;
125, SEQ ID NO;
127, SEQ ID NO;
129, SEQ ID NO;
131, SEQ ID NO;
133, SEQ ID NO;
135, SEQ ID NO;
137, SEQ ID NO;
139, SEQ ID NO;
141, SEQ ID NO;
143, SEQ ID NO;
145, SEQ ID NO;
147, SEQ ID NO;
149, SEQ ID NO;
151, SEQ ID NO;
153, SEQ ID NO;
155, SEQ ID NO;
157 or is a redundant equivalent or fragment thereof;
(k) comprising a purified nucleic acid molecule which hybridizes under high stringency conditions with a nucleic acid molecule according to any one of (h), (i) or
0);
(l) comprising a vector that comprises a nucleic acid molecule according to (h), (i),
0) or (k);
(m) comprising a host cell transformed with a vector according to (1);
(n) comprising a ligand which binds specifically to the Germinal Center Kinase (GCK) subfamily of the STE20 family of protein kinases;
(o) comprising a ligand that binds specifically to a polypeptide according to (a), (b), (c), (d), (e), (f) or (g);
(p) comprising a compound that increases or decreases the level of expression or activity of a polypeptide according to (a), (b), (c), (d), (e), (f) or (g);
(q) comprising a compound that binds to a polypeptide according to (a), (b), (c), (d), (e), (f) or (g) without inducing any of the biological effects of the polypeptide;
(r) comprising a pharmaceutical composition comprising a pharmaceutically acceptable carrier and (i) a polypeptide according to (a), (b), (c), (d), (e), (f) or (g);
or (ii) a polynucleotide according to (h), (i), (j), (k) or (l);
(s) comprising a vaccine composition comprising (i) a polypeptide according to (a), (b), (c), (d), (e), (f) or (g);
or (ii) a polynucleotide according to (h), (i), (j), (k) or (l);
(t) comprising a kit comprising a first container containing a nucleic acid probe that hybridizes under stringent conditions with a nucleic acid molecule according to (h), (i), (j), (k);
a second container containing primers useful for amplifying said nucleic acid molecule; and
instructions for using the probe and primers for facilitating the diagnosis of disease;
(u) comprising the kit of (t), further comprising a third container holding an agent for digesting un-hybridized RNA;
(v) comprising a kit comprising an array of nucleic acid molecules, at least one of which is a nucleic acid molecule according (h), (i),
0), (k);
(w) comprising a kit comprising one or more antibodies that bind to a polypeptide as recited in any one of (a), (b), (c), (d), (e), (f) or (g); and
a reagent useful for the detection of a binding reaction between said antibody and said polypeptide;
or(x) comprising a transgenic or knockout non-human animal that has been transformed to express higher, lower or absent levels of a polypeptide according (a), (b), (c), (d), (e), (f) or (g). - View Dependent Claims (56, 57, 58, 59, 60, 61, 62)
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Specification