Apparatus to automatically lyse a sample
First Claim
1. An apparatus to control temperature while lysing a sample using ultrasonic energy, the apparatus comprising:
- a. a sample container to hold the sample to be lysed;
b. an ultrasonic generator in contact with the sample container to provide ultrasonic energy to the sample;
c. a heat exchanging sub-assembly coupled to the sample container, wherein heat generated within the sample container during application of the ultrasonic energy is transferred from the sample container to the heat exchanging sub-assembly.
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Accused Products
Abstract
A standalone bench top laboratory instrument designed to disrupt, or lyse, cells, spores and tissue samples using ultrasonic energy. The lysing device is programmable, allowing the user control over the sample volume, sonication power level, and lysing duration in order to optimize lysing protocols for specific targets. Once a lysing protocol is entered, the device automatically lyses the sample according to the entered lysing protocol. The lysing device also provides a cooling feature, enabled by a heat exchanging sub-assembly, to prevent the sample from exceeding a maximum set temperature.
61 Citations
37 Claims
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1. An apparatus to control temperature while lysing a sample using ultrasonic energy, the apparatus comprising:
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a. a sample container to hold the sample to be lysed;
b. an ultrasonic generator in contact with the sample container to provide ultrasonic energy to the sample;
c. a heat exchanging sub-assembly coupled to the sample container, wherein heat generated within the sample container during application of the ultrasonic energy is transferred from the sample container to the heat exchanging sub-assembly. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. An apparatus to lyse a sample using ultrasonic energy, the apparatus comprising:
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a. a sample container to hold the sample to be lysed;
b. an ultrasonic generator in contact with the sample container to provide ultrasonic energy to the sample;
c. a heat exchanging sub-assembly coupled to the sample container to facilitate heat transfer to and from the sample container;
d. a variable programming circuit to provide control signals to the ultrasonic generator according to a provided lysing protocol. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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26. An apparatus to lyse a sample using ultrasonic energy, the apparatus comprising:
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a. a sample stored within a sealed environment;
b. a sonication horn positioned to contact an external surface of the sealed environment, wherein the sonication horn generates ultrasonic energy which is provided to the sample via the external surface; and
c. a heat exchanging sub-assembly coupled to the sealed environment to facilitate heat transfer to and from the sealed environment. - View Dependent Claims (27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
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Specification