Device and method for multiple analyte detection
First Claim
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1. A system comprising:
- a device comprising a plurality of detection chambers, channel means in fluid communication with each of the plurality of detection chambers, non-immobilized detection reagents provided in each of the plurality of detection chambers, the non-immobilized detection reagents comprising at least one binding polymer, wherein the non-immobilized detection reagents are effective to selectively bind with a target nucleic acid sequence under conditions effective to produce a detectable fluorescent signal when a target nucleic acid sequence is present in a liquid sample in at least one of the plurality of detection chambers; and
a fluorescence detector for detecting a fluorescent signal, the fluorescence-detector configured for optical communication with one or more of the plurality of detection chambers.
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Abstract
The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.
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Citations
18 Claims
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1. A system comprising:
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a device comprising a plurality of detection chambers, channel means in fluid communication with each of the plurality of detection chambers, non-immobilized detection reagents provided in each of the plurality of detection chambers, the non-immobilized detection reagents comprising at least one binding polymer, wherein the non-immobilized detection reagents are effective to selectively bind with a target nucleic acid sequence under conditions effective to produce a detectable fluorescent signal when a target nucleic acid sequence is present in a liquid sample in at least one of the plurality of detection chambers; and
a fluorescence detector for detecting a fluorescent signal, the fluorescence-detector configured for optical communication with one or more of the plurality of detection chambers. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A device comprising:
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a plurality of detection chambers, channel means in fluid communication with each of the plurality of detection chambers; and
non-immobilized detection reagents provided in each of the plurality of detection chambers, the non-immobilized detection reagents comprising at least one binding polymer, wherein the non-immobilized detection reagents are effective to selectively bind with a target nucleic acid sequence under conditions effective to produce a detectable fluorescent signal when a target nucleic acid sequence is present in a liquid sample in at least one of the plurality of detection chambers. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18)
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Specification