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Directed differentiation of embryonic cells

  • US 20060128013A1
  • Filed: 01/03/2006
  • Published: 06/15/2006
  • Est. Priority Date: 08/01/2000
  • Status: Active Grant
First Claim
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1. A method of directing differentiation of human embryonic cells to a specific differentiated cell type, comprising:

  • a. providing chemically dissociated human embryonic stem (hES) cells;

    b. aggregating the dissociated hES cells in suspension in order to form embryoid bodies;

    c. dissociating the embryoid bodies to provide dissociated single embryonic cells;

    d. culturing said dissociated embryonic cells as a monolayer; and

    e. exposing said embryonic cells to at least one exogenous factor for an effective period of time to direct differentiation of said dissociated embryonic cells to a specific differentiated cell type.

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