Compositions and methods for culturing stem cells
First Claim
1. A method for culturing a stem cell, said method comprising propagating the stem cell in tissue culture medium comprising an agent selected from the group consisting of an inhibitor of a PTPase, a modulator of an enzyme with one or more phosphate binding sites, a phosphohydrolase, a pyrophosphatase, an alkaline phosphatase, an acid phosphatase, and a modulator of a protein with one or more pTyr recognition unit.
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Accused Products
Abstract
The present invention provides methods of culturing, propagating, treating, and maintaining stem cells in the presence of a phosphate mimic. In particular, the invention relates to the propagation of stem cells in vitro, the formation of neurospheres in vitro, and to tissue culture of stem cells in the presence of a phosphate mimic. The invention further relates to the treatment of neurodegenerative disorders.
46 Citations
45 Claims
- 1. A method for culturing a stem cell, said method comprising propagating the stem cell in tissue culture medium comprising an agent selected from the group consisting of an inhibitor of a PTPase, a modulator of an enzyme with one or more phosphate binding sites, a phosphohydrolase, a pyrophosphatase, an alkaline phosphatase, an acid phosphatase, and a modulator of a protein with one or more pTyr recognition unit.
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4. A method for culturing a stem cell, said method comprising propagating the stem cell in tissue culture medium comprising a phosphate mimic.
- 5. A method for culturing a stem cell, said method comprising incubating the stem cells in tissue culture medium comprising a phosphate mimic.
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9. (canceled)
- 10. A method for culturing a stem cell, said method comprising incubating the stem cell in tissue culture medium comprising a phosphate mimic, wherein the amount of phosphate mimic is sufficient for stem cells to exhibit at least 25% more proliferation than stem cells incubated without the phosphate mimic under otherwise same conditions.
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11-13. -13. (canceled)
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14. A method for culturing a neural stem cell, said method comprising incubating the neural stem cell in tissue culture medium comprising a phosphate mimic, wherein the incubating step increases formation of neurospheres from the neural stem cell by at least 25% compared to the formation of neurospheres from the stem cell incubated without phosphate mimic under otherwise same conditions.
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15-16. -16. (canceled)
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18. (canceled)
- 19. A method for culturing a progenitor cell, said method comprising propagating the progenitor cell in tissue culture medium comprising a phosphate mimic.
- 20. A method for culturing a progenitor cell, said method comprising incubating the progenitor cell in tissue culture medium comprising a phosphate mimic.
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23-28. -28. (canceled)
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36. (canceled)
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38-43. -43. (canceled)
Specification