Reaction process

• US 20060128945A1
• Filed: 09/01/2003
• Published: 06/15/2006
• Est. Priority Date: 09/01/2002
• Status: Abandoned Application

• Current Assignee: University of Waikato
• Original Assignee: University of Waikato
• Inventors: Fee, Conan J.
• Application Number:
  US10/526,260
• Publication Number:
  US 20060128945A1
• Time in Patent Office: Days
• US Class Current: 530/350
• CPC Class Codes: B01D 15/34 Size selective separation, e.g. size exclusion chromatography, gel filtration, permeation

Abstract

A reaction process, characterised by the step of introducing reaction components to a medium, wherein at least one of the reaction components has a different flow rate from the other reaction component(s) through the medium, so that a moving reaction phase is formed which causes reaction products.

Claims

1. A reaction process, comprising introducing reaction components to a medium, wherein at least one of the reaction components has a different flow rate from the other reaction component(s) through the medium, so that a moving reaction phase is formed which causes reaction products.

2. The reaction process as claimed in claim 1 further comprising separating the reaction products from the medium.

3. The reaction process as claimed in claim 1 or claim 2 wherein the differences in flow rate through the medium are a consequence of a molecular weight of at least one reaction component.

4. The reaction process as claimed in claim 1 or claim 2 wherein the differences in flow rate through the medium are due to electrostatic charge.

5. The reaction process as claimed in claim 1 or claim 2 wherein the differences in flow rate through the medium are due to ligand interaction.

6. The reaction process as claimed in claim 1 wherein the properties of the medium that provide differing flow rates to the reaction components also leads to separation of the reaction products.

7. The reaction process as claimed in claim 1 wherein the medium includes porous beads.

8. The reaction process as claimed in claim 7 wherein the beads are made of a cross-linked polymeric material.

9. The reaction process as claimed in claim 8 wherein the cross-linked polymeric material includes dextran.

10. The reaction process as claimed in claim 8 wherein the cross-linked polymeric material includes agarose.

11. The reaction process as claimed in claim 1 wherein the moving reaction phase is controlled by altering the properties of the medium.

12. The reaction process as claimed in claim 1 wherein the moving reaction phase is controlled by altering the volumes of the reaction components through a column.

13. The reaction process as claimed in claim 1 wherein the moving reaction phase is controlled by altering the overall flow rate through a column.

14. The reaction process as claimed in claim 1 wherein the reaction products are selectively removed from a reaction zone, preventing them from being involved in subsequent reactions.

15. The reaction process as claimed in claim 14 wherein the reaction products are selectively removed from the reaction zone due to differences in molecular size between the reaction components and the reaction products.

16. The reaction process as claimed in claim 1 wherein properties of the medium are selected to substantially prevent particular reaction products from forming.

17. The reaction process as claimed in claim 16 wherein the properties of the medium are selected to orient the reaction components to provide selectivity of an active site in reactions where multiple active sites exist.

18. The reaction process as claimed in claim 17 wherein said active site is protected through the use of protection chemistry to selectively produce a particular reaction product.

19. The reaction process as claimed in claim 1 wherein the reaction process is used for glycosylation reactions.

20. The reaction process as claimed in claim 1 wherein the reaction process is used for polymerisation.

21. The reaction process as claimed in claim 1 wherein the reaction process is used for cleavage reactions.

22. The reaction process as claimed in claim 1 wherein the reaction process is used for protein PEGylation.

23. The reaction process as claimed in claim 1 wherein the reaction process is controlled using size-exclusion reaction chromatography.

24. A method of protein PEGylation, comprising forming a moving reaction phase using a reaction process as claimed in claim 1.

25. Reaction products produced by the reaction process of claim 1.

26. The reaction products as claimed in claim 25 wherein the products are PEGylated protein.

29. A kitset for use in a reaction process as claimed in claim 1, comprising

unit volumes of at least two reaction components,

a medium,

instructions and

means for bringing two or more reaction components together in a moving reaction phase.

27. (canceled)

28. A kitset to bring two or more reaction components together, comprising

at least two reaction components, and

a medium,

wherein at least one of the reaction components has a different flow rate from the other reaction component(s) through the medium, so that a moving reaction phase is formed, which produces reaction products.

30. (canceled)

31. (canceled)

32. (canceled)