Method of purifying recombinant MSP 1-42 derived from Plasmodium falciparum
First Claim
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1. A method of purifying a merozite surface protein 1 (MSP-1) or a fragment thereof from a feedstream, comprising:
- Solubilizing said merozite surface protein 1 from a feedstream utililizing a tangential flow filtration process;
Washing said filtrate on a membrane with a PBS solution wherein said MSP-1 or fragment thereof remains in the retentate on the purification column;
Adding an aqueous arginine or urea solution to said MSP-1 remaining in the retentate to solubilize it;
Eluting said MSP-1 from said purification column; and
, Purifying out said MSP-1 from elution
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Abstract
Methods of purifying MSP-1 from a variety of source materials including from the milk of transgenic mammals.
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Citations
19 Claims
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1. A method of purifying a merozite surface protein 1 (MSP-1) or a fragment thereof from a feedstream, comprising:
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Solubilizing said merozite surface protein 1 from a feedstream utililizing a tangential flow filtration process;
Washing said filtrate on a membrane with a PBS solution wherein said MSP-1 or fragment thereof remains in the retentate on the purification column;
Adding an aqueous arginine or urea solution to said MSP-1 remaining in the retentate to solubilize it;
Eluting said MSP-1 from said purification column; and
,Purifying out said MSP-1 from elution - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification