Coating solution for glucose sensing membrane and method of manufacturing optical glucose sensor chip

• US 20060134314A1
• Filed: 11/22/2005
• Published: 06/22/2006
• Est. Priority Date: 11/25/2004
• Status: Active Grant

• Current Assignee: Kabushiki Kaisha Toshiba (Toshiba Corporation)
• Original Assignee: Kabushiki Kaisha Toshiba (Toshiba Corporation)
• Inventors: Kasai, Shingo, Tono, Ichiro, Uematsu, Ikuo, Oomiya, Kayoko
• Granted Patent: US 7,651,864 B2
• Time in Patent Office: Days
• US Class Current: 427/2.130
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence
• CPC Class Codes: G01N 33/523 the element being adapted for a specific analyte

Abstract

A method of preparing a coating solution for a glucose sensing membrane, the method comprising preserving a first solution containing an oxidizing enzyme or a reducing enzyme of glucose and a reagent for generating a substance for coloring a coloring agent dissolved in a buffer solution, preserving a second solution containing a mixed solution of an alcohol solution of the coloring agent and a solution of a membrane-forming polymer compound, and mixing the first solution and the second solution.

Claims

1. A method of preparing a coating solution for a glucose sensing membrane, the method comprising:

preserving a first solution containing an oxidizing enzyme or a reducing enzyme of glucose and a reagent for generating a substance for coloring a coloring agent dissolved in a buffer solution;

preserving a second solution containing a mixed solution of an alcohol solution of the coloring agent and a solution of a membrane-forming polymer compound; and

mixing the first solution and the second solution.

2. The method according to claim 1, wherein the first solution is preserved at a temperature lower than the melting point of the solvent of the solution and the second solution is preserved at a temperature of 4 to 10°

C.

3. The method according to claim 1, wherein the oxidizing enzyme is glucose oxidase, the reagent is peroxidase, and the coloring agent is 3,3,5,5-tetramehtylbenzidine.

4. The method according to claim 1, wherein the membrane-forming polymer compound is cellulose type polymer compound.

5. The method according to claim 4, wherein the cellulose type polymer compound is carboxymethyl cellulose or hydroxy cellulose.

6. The method according to claim 1, wherein the second solution further contains an organic compound for making the coating membrane porous.

7. The method according to claim 6, wherein the organic compound is ethylene glycol.

8. A method of manufacturing an optical glucose sensor chip, comprising:

forming a pair of gratings on a glass substrate for receiving and sending light in the substrate; and

forming a glucose sensing membrane by applying the coating solution for a glucose sensing membrane prepared by the method according to claim 1 to a substrate region among the gratings and drying the solution.

9. The method according to claim 8, wherein the surface of gratings is coated with a protection film containing a fluoro resin.

10. The method according to claim 8, wherein the surface treatment is carried out by dry washing with excimer ultraviolet rays and wet treatment with an acid solution before the coating solution for a glucose sensing membrane is applied to the substrate region among the gratings.