Microporous materials, methods, and articles for localizing and quantifying analytes
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Abstract
Described herein are methods for separating one or more analytes present in a fluid sample. The methods involve passing the fluid through a filter having a microporous material, wherein the analytes are localized near the surface of the microporous material. Additional processing steps such as hybridization and amplification can be performed once the analyte is localized. In one method, once the analyte is localized, the analyte can be detected, counted, and correlated in order to determine the concentration of the analyte in the sample. Modified microporous materials and composite materials are also disclosed that can be used in any of the methods and articles described herein. The composite is composed of a microporous material and a pigment, wherein the pigment is incorporated in the microporous material. The pigments alter the optical properties of the microporous material, which enhances the detection of analyte once it is localized. In a further aspect, various kits and articles such as filtration devices containing any of the microporous materials described herein are provided.
87 Citations
193 Claims
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1-131. -131. (canceled)
- 132. A composite comprising a microporous material and a pigment, wherein the pigment is incorporated in the microporous material.
- 172. A method for separating one or more analytes in a fluid sample having a known volume comprising (a) passing the fluid through a filter comprising a microporous material, wherein the analytes are localized near the surface of the microporous material.
- 188. A modified microporous material comprising a microporous material and a suspension polymer, wherein the suspension polymer is localized near the surface of the microporous material.
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191. A filtration device comprising a well body having an inlet and an outlet, wherein the well body has an inner wall and outer wall, and (b) a filter comprising a microporous material capable of localizing an analyte, wherein the filter is attached to the inner wall of the well body.
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192. A filtration device comprising two or more well bodies, wherein each well body comprises a top opening and a bottom opening, and (b) a filter comprising a microporous material capable of localizing an analyte, wherein the filter covers the bottom opening of each well body.
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193. A kit comprising a membrane comprising a microporous material capable of localizing an analyte and a detection means for detecting a labeled nucleic acid.
Specification