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Microfluidic device for cell separation and uses thereof

  • US 20060134599A1
  • Filed: 09/29/2003
  • Published: 06/22/2006
  • Est. Priority Date: 09/27/2002
  • Status: Active Grant
First Claim
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1. A method for producing a population enriched in a first type of cell from a mixture of at least three types of cells, said method comprising the steps of:

  • (a) providing a mixture of cells comprising at least first, second, and third types of cells;

    (b) contacting said mixture with a first microfluidic channel that selectively allows the passage therethrough of said first type of cell to produce a first cell population enriched in said first type of cells relative to said second type of cell; and

    (c) contacting said first cell population with a second microfluidic channel that selectively allows the passage therethrough of said first type of cell to produce a second cell population enriched in said first type of cell relative to said second and third types of cells, thereby producing said population enriched in said first type of cell.

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