Method for the simultaneous detection of HPV RNA and DNA in a sample

• US 20060134615A1
• Filed: 12/08/2005
• Published: 06/22/2006
• Est. Priority Date: 12/20/2004
• Status: Abandoned Application

• Current Assignee: Cytyc Corporation (Hologic Incorporated)
• Original Assignee: Cytyc Corporation (Hologic Incorporated)
• Inventors: Linder, James, Lentrichia, Brian, Burg, J. Lawrence
• Application Number:
  US11/297,689
• Publication Number:
  US 20060134615A1
• Time in Patent Office: Days
• US Class Current: 435/5
• CPC Class Codes: C12Q 1/70 involving virus or bacteriophage immunoassay for viruses G01N33/56983
• CPC Class Codes: C12Q 1/708 for papilloma

Abstract

A method is provided for the simultaneous detection of human papillomavirus DNA and RNA in a sample.

Claims

1. A method for the simultaneous detection of human papiloma virus (HPV) DNA and RNA sequences in a sample, the method comprising;

a) obtaining a sample of biological material containing a target nucleotide sequence and isolating total nucleic acid from said sample

b) detecting the presence of HPV DNA and RNA simultaneously in said sample

wherein the presence of HPV DNA is indicative of HPV in said sample and the presence of HPV RNA is indicative of an active HPV infection in said sample.

2. The method of claim 1, wherein sample is comprised of cervical cells.

3. The method of claim 1, wherein the presence of HPV DNA and RNA is detected by amplifying a target nucleic acid sequence in said sample.

4. The method of claim 1, wherein the presence of HPV DNA and RNA is detected by the binding of a probe to a target nucleic acid sequence in said sample.