Biosensor apparatus and methods of use
First Claim
1. A biosensor for use in detecting a target analyte in a fluid sample, the device comprising:
- a reaction chamber including an immobilized binding site and a probe conjugate, wherein the probe conjugate comprises a binding partner adapted to bind to the immobilized binding site;
a detection chamber including electrodes for detecting an electrochemical reaction in the detection chamber; and
a fluid passageway between the reaction chamber and the detection chamber, wherein, the presence or absence of a target analyte in the fluid sample results in a change in the amount of probe conjugate bound in the reaction chamber, the change detectable with an electrochemical reaction in the detection chamber.
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Abstract
Disclosed herein are methods and devices for detecting the presence of an analyte of interest. A biosensor device can include a reaction chamber and an electrochemical detection chamber. The reaction chamber can include at least one immobilized binding site and a probe conjugate adapted to bind to at least one of the target analyte and the immobilized binding site, while the detection chamber can include electrodes for detecting an electrochemical reaction. If present, the target analyte in the fluid sample results in a change in the amount of probe conjugate bound in the reaction chamber, which can be detected electrochemically in the detection chamber.
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Citations
24 Claims
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1. A biosensor for use in detecting a target analyte in a fluid sample, the device comprising:
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a reaction chamber including an immobilized binding site and a probe conjugate, wherein the probe conjugate comprises a binding partner adapted to bind to the immobilized binding site;
a detection chamber including electrodes for detecting an electrochemical reaction in the detection chamber; and
a fluid passageway between the reaction chamber and the detection chamber, wherein, the presence or absence of a target analyte in the fluid sample results in a change in the amount of probe conjugate bound in the reaction chamber, the change detectable with an electrochemical reaction in the detection chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. An biosensor for use in detecting a target antigen in a fluid sample, the device comprising:
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a reaction chamber including at least one immobilized binding site including a antibody and a probe conjugate including an antibody adapted to bind to bound target antigen;
a detection chamber including electrodes for detecting an electrochemical reaction in the detection chamber; and
a fluid passageway between the reaction chamber and the detection chamber, wherein, the presence of a target antigen in the fluid sample results in a reduction in the amount of probe conjugate bound in the reaction chamber, the reduction being detectable with an electrochemical reaction in the detection chamber.
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19. A method of detecting a target analyte in a fluid sample, the method comprising the steps of:
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delivering a sample to a biosensor that includes a reaction chamber and a detection chamber;
allowing a reaction to proceed in the reaction chamber between an immobilized binding site and a probe conjugate;
moving the sample into a detection chamber and electrochemically detecting the level of probe conjugate, wherein the presence of a target analyte in the sample results in an increase or a decrease in the amount of probe conjugate detected in the reaction chamber. - View Dependent Claims (20, 21, 22, 23, 24)
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Specification