Electrospun enzyme-nanocomposite biosensing material
First Claim
1. A biosensing material comprising polymer-enzyme nanocomposite fibers wherein the polymer-enzyme nanocomposite fibers retain enzyme activity.
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Abstract
The present invention provides biosensing material comprising polymer-enzyme nanocomposite fibers. A biosensor comprising such material may be obtained through an electrospinning process to yield a nonwoven mat, which retains enzyme activity. The large amount of available surface area obtained by the methods of the present invention provides unusually high sensitivity and fast response time in sensing applications. Also provided is a biosensing material for monitoring the concentration of an analyte present in a sample, such as urea. The biosensing material contains nanocomposite fibers of an enzyme, such as urease and at least one polymer produced through an electrospinning process. If desired, the enzyme may be encapsulated inside metal oxide semiconductor thin films. A method for preparing the biosensing material through an electrospinning technique is also provided.
16 Citations
21 Claims
- 1. A biosensing material comprising polymer-enzyme nanocomposite fibers wherein the polymer-enzyme nanocomposite fibers retain enzyme activity.
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6. A method of producing a biosensor comprising:
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injecting a solution that comprises at least one polymer, an enzyme and a buffer under the influence of an electric field wherein the build-up of electrostatic charges on a surface of a liquid droplet of the solution induces the formation of a jet;
stretching the induced jet formed by the build-up of electrostatic charges on the surface of the liquid droplet of the solution to form at least one continuous fiber; and
collecting the at least one continuous fiber on a conductor surface to form a nonwoven mat which retains enzyme activity and has a high surface area with a relatively small pore size. - View Dependent Claims (7, 8, 9, 10, 11, 21)
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Specification