Magnetism based rapid cell separation
First Claim
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1. A process for isolating a target cell, cellular organelle or virus from a sample, which process comprises:
- a) contacting a sample containing or suspected of containing a target cell, cellular organelle or virus with a magnetic microbead, said magnetic microbead not comprising a moiety that binds to said target cell, cellular organelle or virus with high specificity;
b) allowing said target cell, cellular organelle or virus, if present in said sample, to bind to said magnetic microbead nonspecifically or with low specificity to form a conjugate between said target cell, cellular organelle or virus and said magnetic microbead; and
c) separating said conjugate from other undesirable constituents via a magnetic force to isolate said target cell, cellular organelle or virus from said sample.
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Abstract
This invention relates generally to the field of. In particular, the invention provides processes and kits for isolating a target cell, cellular organelle or virus from a sample, using inter alia, non- or low-specific binding between a target cell, cellular organelle or virus with a magnetic microbead.
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Citations
55 Claims
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1. A process for isolating a target cell, cellular organelle or virus from a sample, which process comprises:
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a) contacting a sample containing or suspected of containing a target cell, cellular organelle or virus with a magnetic microbead, said magnetic microbead not comprising a moiety that binds to said target cell, cellular organelle or virus with high specificity;
b) allowing said target cell, cellular organelle or virus, if present in said sample, to bind to said magnetic microbead nonspecifically or with low specificity to form a conjugate between said target cell, cellular organelle or virus and said magnetic microbead; and
c) separating said conjugate from other undesirable constituents via a magnetic force to isolate said target cell, cellular organelle or virus from said sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41)
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42. A kit for isolating a target cell, cellular organelle or virus from a sample, which kit comprises in a same or different container(s):
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a) a magnetic microbead for contacting a sample containing or suspected of containing a target cell, cellular organelle or virus, said magnetic microbead not comprising a moiety that binds to said target cell, cellular organelle or virus with high specificity;
b) means for allowing said target cell, cellular organelle or virus, if present in said sample, to bind to said magnetic microbead nonspecifically or with low specificity to form a conjugate between said target cell, cellular organelle or virus and said magnetic microbead; and
c) means for separating said conjugate from other undesirable constituents via a magnetic force to isolate said target cell, cellular organelle or virus from said sample. - View Dependent Claims (43)
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44. A process for isolating a virus or bacteriophage from a sample, which process comprises:
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a) removing cells from a sample containing or suspected of containing a target virus or bacteriophage;
b) contacting said cell-free sample with a magnetic microbead, said magnetic microbead not comprising a moiety that binds to said target virus or bacteriophage with high specificity;
c) allowing said target virus or bacteriophage, if present in said sample, to bind to said magnetic microbead nonspecifically or with low specificity to form a conjugate between said target virus or bacteriophage and said magnetic microbead; and
c) separating said conjugate from other undesirable constituents via a magnetic force to isolate said target virus or bacteriophage from said sample. - View Dependent Claims (45, 46, 47, 48, 49, 50, 51, 52, 53, 54)
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55. A kit for isolating a virus or bacteriophage from a sample, which kit comprises in a same or different container(s):
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a) means for removing cells from a sample containing or suspected of containing a target virus or bacteriophage;
b) a magnetic microbead for contacting said cell-free sample, said magnetic microbead not comprising a moiety that binds to said target cell, cellular organelle or virus with high specificity;
c) means for allowing said target virus or bacteriophage, if present in said cell-free sample, to bind to said magnetic microbead nonspecifically or with low specificity to form a conjugate between said target virus or bacteriophage and said magnetic microbead; and
d) means for separating said conjugate from other undesirable constituents via a magnetic force to isolate said target virus or bacteriophage from said sample.
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Specification