Porous substrates and arrays comprising the same
First Claim
1. An array comprising at least one substrate support and a plurality of discrete regions, each said discrete region comprising a porous substrate attached to or supported by said at least one substrate support, and said porous substrate being accessible from at least two sides of said array.
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Accused Products
Abstract
The present invention relates to arrays comprising porous substrates for attachment of nucleic acids, polypeptides, membranes, or other biological or organic materials. In many embodiments, the arrays of the present invention have a flow-through configuration such that washing buffers or samples can access to the porous substrates from at least two sides of the arrays. The present invention also features arrays comprising UV-compatible porous substrates, arrays comprising three-dimensional membranes in sol-gels, and arrays comprising silica-based porous substrates prepared using a low-temperature fusion process.
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Citations
51 Claims
- 1. An array comprising at least one substrate support and a plurality of discrete regions, each said discrete region comprising a porous substrate attached to or supported by said at least one substrate support, and said porous substrate being accessible from at least two sides of said array.
- 17. An array comprising a substrate support including a plurality of discrete regions, each said discrete region comprising a porous substrate which is a fusion product of a mixture comprising silica beads and at least one silane.
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25. A method of fabricating an array, comprising the steps of:
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formulating silica beads in an organic solvent comprising said at least one silane;
depositing said formulated silica beads in discrete regions of a substrate support; and
fusing said silica beads to form porous substrates in said discrete regions.
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- 26. An array comprising a substrate support including a plurality of discrete regions, each of which comprises a gelation product of a mixture comprising at least one sol-gel precursor and a membrane.
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31. A method for fabricating an array, comprising the steps of:
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mixing at least one sol-gel precursor with a membrane under conditions that no significant gelation occurs;
depositing said mixed sol-gel precursor and membrane into discrete regions on a substrate support; and
initiating gelation in each of said discrete regions to form a sol-gel including said membrane.
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32. A method for fabricating an array, comprising the steps of:
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mixing at least one sol-gel precursor with a membrane;
hydrolyzing said at least one sol-gel precursor to form a sol-gel including said membrane; and
deposit said sol-gel into discrete regions of a substrate support.
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- 33. An array comprising a substrate support including a plurality of discrete regions, each of which comprises a UV-compatible porous substrate.
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47. A silica-based porous flow-through microplate fabricated according to the following steps:
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producing a plurality of channels by sand blasting or laser drilling at predetermined locations on a glass plate;
depositing patches of silicate frits to said predetermined locations;
sintering to consolidate said frits to form porous substrates; and
assembling the glass plate into a microplate.
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48. A flow-through microplate fabricated according to the following steps:
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producing a plurality of channels by injection molding at predetermined regions on a substrate support;
reformulating silicate frits with silanes;
depositing patches of sol-gels containing said silicate frits and silanes to the predetermined regions on the substrate support;
consolidating said silicate frits and silanes to form porous substrates; and
assembling the substrate support to form a microplate.
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49. A stand-alone porous disc-based microplate fabricated according to the following steps:
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injection molding to make a holey microplate, said holey microplate comprising recess areas in predetermined regions on a side wall of a well of said holey microplate;
depositing patches of silicate frits to a substrate support;
consolidating the silicate frits to form standalone porous substrates; and
positioning the standalone porous substrates into the recess areas of the holey microplate.
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50. A flow-through polymeric microplate comprising polymeric porous substrates, said polymeric microplate being fabricated according to the following steps:
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producing channels by injection molding at predetermined locations on a polymeric substrate support;
positioning polymeric porous substrates to the predetermined locations; and
assembling the polymeric substrate support and the polymeric porous substrates to form a microplate by thermal bonding or adhesive chemistry.
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51. A flow-through microplate prepared according to the following steps:
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positional etching of a glass substrate to form separate porous substrate patches at predetermined locations such that only a top layer of the glass substrate becomes porous;
sand blasting or laser drilling to prepare at least one channel underneath each said porous substrate patch at the predetermined locations such that the channel passes through the substrate; and
assembling the substrate to form a microplate.
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Specification