Method for creating a reference region and a sample region on a biosensor and the resulting biosensor

• US 20060141527A1
• Filed: 12/29/2004
• Published: 06/29/2006
• Est. Priority Date: 12/29/2004
• Status: Abandoned Application

• Current Assignee: Corning Incorporated
• Original Assignee: Corning Incorporated
• Inventors: Peng, Jinlin, Frutos, Anthony G., Piech, Garrett A., Webb, Michael B., Caracci, Stephen J.
• Application Number:
  US11/027,509
• Publication Number:
  US 20060141527A1
• Time in Patent Office: Days
• US Class Current: 435/7.100
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00315 Microtiter plates
• CPC Class Codes: B01J 2219/00367 capillary
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00382 Stamping
• CPC Class Codes: B01J 2219/00385 Printing
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00662 Two-dimensional arrays within two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00693 Means for quality control
• CPC Class Codes: B01J 2219/00722 : Nucleotides
• CPC Class Codes: B01J 2219/00725 : Peptides
• CPC Class Codes: B01L 2200/12 : Specific details about manufacturing devices
• CPC Class Codes: B01L 2200/148 : Specific details about calibrations
• CPC Class Codes: B01L 2300/0636 : Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0829 : Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/5085 : for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 2035/00158 : Elements containing microarrays, i.e. "biochip"
• CPC Class Codes: G01N 21/553 : and using surface plasmons fluorescence excitation G01N21/648; enhanced Raman G01N21/658
• CPC Class Codes: G01N 21/7743 : the reagent-coated grating coupling light in or out of the waveguide

Abstract

A method is described herein that can use any one of a number of deposition techniques to create a reference region and a sample region on a single biosensor which in the preferred embodiment is located within a single well of a microplate. The deposition techniques that can be used to help create the reference region and the sample region on a surface of the biosensor include: (1) the printing/stamping of a deactivating agent on a reactive surface of the biosensor; (2) the printing/stamping of a target molecule (target protein) on a reactive surface of the biosensor; or (3) the printing/stamping of a reactive agent on an otherwise unreactive surface of the biosensor.

Claims

1. A biosensor that has a surface comprising a reference region and a sample region which were created in part by using a deposition technique.

2. The biosensor of claim 1, wherein the reference region and the sample region were created on said surface by performing the following steps:

coating said surface with a reactive agent;

depositing a deactivating agent on a predetermined area of said coated surface to create the reference region; and

exposing the surface to target molecules wherein the target molecules bind to the surface in a defined area of said coated surface that was not treated with deactivating agent to create the sample region.

3. The biosensor of claim 1, wherein the reference region and the sample region were created on said surface by performing the following steps:

coating said surface with a reactive agent;

depositing target molecules on a predetermined area of said coated surface to create the sample region; and

exposing said coated surface to a deactivating agent to inactivate a portion of said coated surface that still has the reactive agent exposed thereon to create the reference region.

4. The biosensor of claim 1, wherein the reference region and the sample region were created on said surface by performing the following steps:

depositing an activating agent on a predetermined area of said surface and attaching target molecules to at least a portion of said coated surface that has the activating agent exposed thereon to create the sample region; and

using the region without the activating agent as the reference region.

5. The biosensor of claim 1, wherein said surface includes more than one reference region and/or more than one sample region.

6. The biosensor of claim 1, wherein said surface which includes the reference region and the sample region enables one to use the sample region to detect the biomolecular binding event and also enables one to use the reference region to reference out effects that can adversely affect the detection of the biomolecular binding event.

7. The biosensor of claim 1, wherein said surface which includes the reference region and the sample region enables one to use mass spectrometry to detect both regions to obtain further information about a biological binding event.

8. The biosensor of claim 1, wherein said reference region is created by depositing molecules which resist the non-specific binding of target molecules.

9. The biosensor of claim 1, wherein said surface is located in a bottom of a well in a microplate.

10. The biosensor of claim 1, wherein said surface is a slide.

11. The biosensor of claim 1, wherein said biosensor is a surface plasmon resonance sensor.

12. The biosensor of claim 1, wherein said biosensor is a resonant waveguide grating sensor.

13. The biosensor of claim 1, wherein said deposition technique is contact pin printing.

14. The biosensor of claim 1, wherein said deposition technique is non-contact printing like ink jet printing or aerosol printing.

15. The biosensor of claim 1, wherein said deposition technique is capillary printing.

16. The biosensor of claim 1, wherein said deposition technique is microcontact printing.

17. The biosensor of claim 1, wherein said deposition technique is pad printing.

18. The biosensor of claim 1, wherein said deposition technique is screen printing.

19. The biosensor of claim 1, wherein said deposition technique is silk screening.

20. The biosensor of claim 1, wherein said deposition technique is micropipetting.

21. The biosensor of claim 1, wherein said deposition technique is spraying.

22. A microplate comprising:

a frame including a plurality of wells formed therein, each well incorporating a biosensor that has a surface with a reference region and a sample region which were created in part by using a deposition technique.

23. The microplate of claim 22, wherein the reference region and the sample region were created on said surface by performing the following steps:

coating said surface with a reactive agent;

depositing a deactivating agent on a predetermined area of said coated surface to create the reference region; and

exposing the surface to target molecules wherein the target molecules bind to the surface in a defined area of said coated surface that was not treated with deactivating agent to create the sample region.

24. The microplate of claim 22, wherein the reference region and the sample region were created on said surface by performing the following steps:

coating said surface with a reactive agent;

depositing target molecules on a predetermined area of said coated surface to create the sample region; and

exposing said coated surface to a deactivating agent to inactivate a portion of said coated surface that still has the reactive agent exposed thereon to create the reference region.

25. The microplate of claim 22, wherein the reference region and the sample region were created on said surface by performing the following steps:

depositing an activating agent on a predetermined area of said surface and attaching target molecules to at least a portion of said coated surface that has the activating agent exposed thereon to create the sample region; and

using the region without the activating agent as the reference region.

26. The microplate of claim 22, wherein said surface includes more than one reference region and/or more than one sample region within each well.

27. The microplate of claim 22, wherein said biosensor which has the reference region and the sample region enables one to use the sample region to detect a biomolecular binding event and also enables one to use the reference region to reference out spurious changes that can adversely affect the detection of the biomolecular binding event.

28. The microplate of claim 22, wherein said biosensor is a surface plasmon resonance sensor.

29. The microplate of claim 22, wherein said biosensor is a resonant waveguide grating sensor.

30. The microplate of claim 22, wherein said deposition technique includes one of the following:

contact pin printing, non-contact printing (ink jet printing, aerosol printing), capillary printing, microcontact printing, pad printing, and screen printing, silk screening, micropipetting, and spraying.

31. A method for preparing a patterned surface on a biosensor, said method comprising the step of:

utilizing a deposition technique to create a reference region and a sample region on the surface of said biosensor.

32. The method of claim 31, wherein the reference region and the sample region are created on the surface of said biosensor by performing the following steps:

coating said surface with a reactive agent;

depositing a deactivating agent on a predetermined area of said coated surface to create the reference region; and

exposing the surface to target molecules wherein the target molecules bind to the surface in a defined area of said coated surface that was not treated with deactivating agent to create the sample region.

33. The method of claim 31, wherein the reference region and the sample region are created on the surface of said biosensor by performing the following steps:

coating said surface with a reactive agent;

depositing target molecules on a predetermined area of said coated surface to create the sample region; and

exposing said coated surface to a deactivating agent to inactivate a portion of said coated surface that still has the reactive agent exposed thereon to create the reference region.

34. The method of claim 31, wherein the reference region and the sample region are created on the surface of said biosensor by performing the following steps:

depositing an activating agent on a predetermined area of said surface and attaching target molecules to at least a portion off said coated surface that has the activating agent exposed thereon to create the sample region; and

using the region without the activating agent as the reference region.

35. The method of claim 31, wherein said biosensor F has more than one reference region and/or more than one sample region.

36. The method of claim 31, wherein said biosensor which has the reference region and the sample region enables one to use the sample region to detect a biomolecular binding event and also enables one to use the reference region to reference out spurious changes that can adversely affect the detection of the biomolecular binding event.

37. The method of claim 31, wherein said biosensor is located in a bottom of a well in a microplate.

38. The method of claim 31, wherein said biosensor is a surface plasmon resonance sensor.

39. The method of claim 31, wherein said biosensor is a resonant waveguide grating sensor.

40. The method of claim 31, wherein said deposition technique includes one of the following:

contact pin printing, non-contact printing (ink jet printing, aerosol printing), capillary printing, microcontact printing, pad printing, and screen printing, silk screening, micropipetting, and spraying.