Capillary perfused bioreactors with multiple chambers
First Claim
1. A bioreactor comprising:
- i. a first substrate having a first surface, an opposite second surface and edges;
ii. a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, wherein the bottom surface is located therebetween the first surface and the second surface, and wherein the first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium;
iii. a recess formed in the second substrate with a bottom surface and in fluid communication with the channel; and
iv. a barrier positioned for covering the recess so as to form an outer chamber, wherein the barrier has a porosity to allow the channel and the outer chamber in fluid communication and control the move of at least one predetermined type of cells between the channel and the outer chamber.
2 Assignments
0 Petitions
Accused Products
Abstract
A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium. In forming the bioreactor, the channel is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel. The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel.
-
Citations
57 Claims
-
1. A bioreactor comprising:
-
i. a first substrate having a first surface, an opposite second surface and edges;
ii. a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, wherein the bottom surface is located therebetween the first surface and the second surface, and wherein the first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium;
iii. a recess formed in the second substrate with a bottom surface and in fluid communication with the channel; and
iv. a barrier positioned for covering the recess so as to form an outer chamber, wherein the barrier has a porosity to allow the channel and the outer chamber in fluid communication and control the move of at least one predetermined type of cells between the channel and the outer chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
-
-
28. A bioreactor comprising:
-
i. a first substrate having a first surface and an opposite second surface, defining a chamber therebetween for receiving cells and a liquid medium;
ii. an inlet port formed in the first substrate;
iii. a first connection channel formed in the first substrate, wherein the first connection channel is in fluid communication with the inlet port and the chamber for allowing a stream of substance to be delivered to the chamber;
iv. an outlet port formed in the first substrate;
v. a second connection channel formed in the first substrate, wherein the second connection channel is in fluid communication with the outlet port and the chamber for allowing a stream of substance to be removed from the chamber; and
vi. confining means positioned in a region in the chamber proximate to the first connection channel to confine the cells. - View Dependent Claims (29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41)
-
-
42. A bioreactor comprising:
-
i. a first substrate having a first surface and an opposite second surface, defining a chamber therebetween for receiving cells and a liquid medium;
ii. an inlet port formed in the first substrate;
iii. a first connection channel formed in the first substrate, wherein the first connection channel is in fluid communication with the inlet port and the chamber for allowing a stream of substance to be delivered to the chamber;
iv. an outlet port formed in the first substrate;
v. a second connection channel formed in the first substrate, wherein the second connection channel is in fluid communication with the outlet port and the chamber for allowing a stream of substance to be removed from the chamber, and vi. confining means positioned in the chamber to form a confinement region to confine the cells therein. - View Dependent Claims (43, 44, 45, 46, 47, 48, 49)
-
-
50. A bioreactor comprising:
-
i. a first substrate having a first surface and an opposite second surface, defining a first chamber therebetween for receiving a first type of cells and a liquid medium;
ii. at least one second chamber formed in the first substrate for receiving a second type of cells and a liquid medium;
iii. at least one connection channel formed in the first substrate, wherein at least one connection channel is in fluid communication with a corresponding second chamber and the first chamber for allowing the first type of cells and the second type of the cells to interact with each other. - View Dependent Claims (51, 52, 53, 54, 55, 56, 57)
-
Specification