Apparatus and method to elute microorganisms from a filter

• US 20060141636A1
• Filed: 12/16/2005
• Published: 06/29/2006
• Est. Priority Date: 12/16/2004
• Status: Abandoned Application

• Current Assignee: IDEXX Laboratories Incorporated
• Original Assignee: IDEXX Laboratories Incorporated
• Inventors: Steggles, Darron, Brewin, Brett
• Application Number:
  US11/303,531
• Publication Number:
  US 20060141636A1
• Time in Patent Office: Days
• US Class Current: 436/177
• CPC Class Codes: C12M 33/14 with filters, sieves or membranes
• CPC Class Codes: G01N 1/34 Purifying; Cleaning processes or apparatus for extracting or separating nucleic acids from biological samples C12N15/1003
• CPC Class Codes: G01N 1/4077 by other techniques involving separation of suspended solids
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

There is provided apparatuses and methods for eluting microorganisms from filter media. The apparatus includes a housing for receiving filer media suspected of containing microorganisms and means for exposing the filter media to a pressurized buffer solution. By passing the buffer solution through the filter media under pressure, microorganisms trapped in or on the filter media are eluted therefrom.

Claims

1. An apparatus for eluting microorganisms from filter media comprising:

a housing configured and dimensioned to receive filter media, the housing having an inlet and an outlet;

filter media disposed in the housing, the filter media having been exposed to a liquid suspected of containing microorganisms;

means for transporting a liquid buffer solution into the housing via the outlet; and

means for causing the liquid buffer solution to pass through the filter media under pressure and to exit the housing via the inlet.

2. The apparatus according to claim 1, wherein the means for causing the fluid buffer solution to pass through the filter media include a pressurizing assembly selectively connectable to the outlet of the housing.

3. The apparatus according to claim 2, wherein the pressurizing assembly includes a pressure chamber configured for pressurizing a quantity of a liquid buffer solution therein prior to transportation of the liquid buffer solution to the housing.

4. The apparatus according to claim 3, wherein the pressure chamber is in selective fluid communication with a source of pressurizing gas.

5. The apparatus according to claim 4, wherein the pressurizing assembly includes an air valve fluidly disposed between the source of pressurizing gas and the pressure chamber and a non-return valve fluidly disposed between the air valve and the pressure chamber.

6. The apparatus according to claim 2, further comprising a reservoir configured to store a quantity of a liquid buffer solution therein, and a first conduit in fluid communication with the reservoir, wherein the first conduit includes a free end configured to selectively fluidly connect with the pressure chamber.

7. The apparatus according to claim 6, further comprising a liquid buffer solution contained within the reservoir.

8. The apparatus according to claim 7, further comprising a buffer inlet valve fluidly disposed between the reservoir and the pressure chamber.

9. The apparatus according to claim 8, further comprising an elution valve fluidly connected to the pressure chamber and fluidly connectable to the outlet of the housing.

10. The apparatus according to claim 9, further comprising a venting valve fluidly connected to the pressure chamber.

11. The apparatus according to claim 10, wherein the pressure chamber is pressurizable to a pressure of between about 0 psi (0 Bars) to at least about 72.5 psi (5.0 Bars).

12. The apparatus according to claim 1, wherein the filter media includes a plurality of discs stacked upon one another, wherein the stack of discs alternate between relatively large outer diameter discs and relatively small outer diameter discs, and wherein the stack of discs is compressed in a linear direction.

13. A method for eluting microorganisms from filter media comprising the steps of:

providing filter media suspected of containing microorganisms; and

forcing a pressurized liquid through the filter media to at least partially elute microorganisms from the filter media, if present.

14. The method according to claim 13, wherein the step of forcing a pressurized liquid through the filter media includes forcing the pressurized liquid through the filter media in a direction opposite to a direction of filtration.

15. The method according to claim 14, further comprising the step of forcing a fixed quantity of pressurized liquid at a known initial pressure through the filter media.

16. The method according to claim 15, further comprising the step of providing an apparatus for eluting the filter media;

the apparatus including;

a pressurizing assembly selectively connectable to the outlet of the housing, wherein the pressurizing assembly includes a pressure chamber configured for pressurizing a quantity of a liquid buffer solution therein prior to transportation of the liquid buffer solution to the housing;

a source of pressurizing gas in selective fluid communication with the pressure chamber;

an air valve fluidly disposed between the source of pressurizing gas and the pressure chamber and a non-return valve fluidly disposed between the air valve and the pressure chamber;

a reservoir configured to store a quantity of a liquid buffer solution therein, and a first conduit in fluid communication with the reservoir, wherein the first conduit includes a free end configured to selectively fluidly connect with the pressure chamber;

a liquid buffer solution contained within the reservoir;

a buffer inlet valve fluidly disposed between the reservoir and the pressure chamber;

an elution valve fluidly connected to the pressure chamber and fluidly connectable to the outlet of the housing; and

a venting valve fluidly connected to the pressure chamber.

17. The method according to claim 16, further comprising the step of:

introducing a fixed quantity of liquid buffer solution to the pressure chamber.

18. The method according to claim 17, further comprising the step of pressurizing the pressure chamber a pressure of between about 0 psi (0 Bars) to at least about 72.5 psi (5.0 Bars).

19. The method according to claim 18, further comprising the step of:

forcing the pressurized liquid buffer solution through the filter media.