Analysis apparatus and method comprising auto-focusing means

• US 20060142662A1
• Filed: 06/15/2004
• Published: 06/29/2006
• Est. Priority Date: 06/19/2003
• Status: Abandoned Application

• Current Assignee: Koninklijke Philips Electronics N.V. (Koninklijke Philips N.V.)
• Original Assignee: Koninklijke Philips Electronics N.V. (Koninklijke Philips N.V.)
• Inventors: Van Beek, Michael Cornelis
• Application Number:
  US10/560,453
• Publication Number:
  US 20060142662A1
• Time in Patent Office: Days
• US Class Current: 600/476
• CPC Class Codes: A61B 2562/0242 for varying or adjusting the optical path length in the tissue
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 5/0068 Confocal scanning
• CPC Class Codes: A61B 5/0075 by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy A61B5/0071 takes precedence
• CPC Class Codes: A61B 5/14546 for measuring analytes not otherwise provided for, e.g. ions, cytochromes
• CPC Class Codes: A61B 5/441 Skin evaluation, e.g. for skin disorder diagnosis
• CPC Class Codes: A61B 5/489 Blood vessels
• CPC Class Codes: G01N 21/4795 spatially resolved investigating of object in scattering medium in vivo A61B
• CPC Class Codes: G01N 21/65 Raman scattering

Abstract

The present invention relates to an analysis apparatus, in particular a spectroscopic analysis apparatus, for analyzing an object, such as blood of a patient, and a corresponding analysis method. To aim the confocal detection volume inside a blood vessel orthogonal polarized spectral imaging (OPS imaging) is used to locate blood capillaries in the skin. Image processing means (ipm) determining image characteristics, which indicate if an imaging system (img) for imaging the object is focused on the object (obj) to be analyzed, from a detected image. Said image processing means (ipm) are preferably adapted for determining the amplitudes of spatial frequencies corresponding to typical characteristics of the object (obj) from a detected image, or for determining the maximum contrast present in a detected image. Based on the determined image characteristics, auto-focusing means (afm) control the focusing means (mo) to change the focusing accordingly, whereafter the object is imaged and the same image characteristics are determined again from a the new image. This is preferably repeatedly done until the object (obj) substantially lies in a detection plane (dp) onto which the focusing means (mo) are focused. Thus, continuous autofocusing with high accuracy can be achieved.

Claims

1. An analysis apparatus, in particular a spectroscopic analysis apparatus, for analyzing an object comprising:

an excitation system for emitting an excitation beam to excite a target region,

a monitoring system comprising a monitoring beam source for emitting a monitoring beam and an imaging system to image the target region,

a detection system for detecting scattered radiation from the target region generated by the excitation beam,

focusing means for focusing the excitation system, the monitoring system and the detection system on a detection plane in the target region,

image processing means for determining image characteristics, which indicate if the imaging system is focused on the object to be analyzed, from a detected image, and

auto-focusing means for controlling the focusing means to change the focusing of the monitoring system, the excitation system and the detection system based on the determined image characteristics, for controlling the monitoring system to image the target region and for controlling the image processing means to determine the image characteristics from a detected image until the object substantially lies in the detection plane.

2. An analysis apparatus as claimed in claim 1, wherein said image processing means are adapted for determining the amplitudes of spatial frequencies corresponding to typical characteristics of the object from a detected image and wherein said auto-focusing means are adapted for controlling the focusing means to change the focusing of the monitoring system, the excitation system and the detection system based on the determined image characteristics, for controlling the monitoring system to repeatedly image the target region and for controlling the image processing means to determine the image characteristics from a detected image until the determined amplitudes of spatial frequencies are maximally.

3. An analysis apparatus as claimed in claim 2, wherein said analysis apparatus is adapted for in vivo analysis of blood and wherein said image processing means are adapted for determining the amplitudes of spatial frequencies corresponding to typical diameters of blood vessels from a detected image.

4. An analysis apparatus as claimed in claim 1, wherein said image processing means are adapted for determining the maximum contrast present in a detected image and/or at one or more image portions corresponding to the object or object portions and wherein said auto-focusing means are adapted for controlling the focusing means to change the focusing of the monitoring system, the excitation system and the detection system based on the determined image characteristics, for controlling the monitoring system to repeatedly image the target region and for controlling the image processing means to determine the image characteristics from a detected image until the determined contrast is maximally.

5. An analysis apparatus as claimed in claim 4, wherein said analysis apparatus is adapted for in vivo analysis of blood and wherein said image processing means are adapted for determining the maximum contrast present in a detected image between blood and surrounding tissue, in particular at the edges of blood vessels.

6. An analysis apparatus as claimed in claim 4, wherein said auto-focusing means are adapted for controlling the focusing means to change the focusing of the monitoring system, the excitation system and the detection system based on the determined image characteristics, for controlling the monitoring system to repeatedly image the target region and for controlling the image processing means to determine the image characteristics from a detected image until the determined the intensity of one or more pixels in the detected image show an extremum.

7. An analysis apparatus as claimed in claim 4, wherein said auto-focusing means are adapted for controlling the focusing means to change the focusing of the monitoring system, the excitation system and the detection system based on the determined image characteristics, for controlling the monitoring system to repeatedly image the target region and for controlling the image processing means to determine the image characteristics from a detected image until the spread in intensity of pixels in the detected image is maximally.

8. An analysis apparatus as claimed in claim 4, wherein said auto-focusing means are adapted for controlling the focusing means to change the focusing of the monitoring system, the excitation system and the detection system based on the determined image characteristics, for controlling the monitoring system to repeatedly image the target region and for controlling the image processing means to determine the image characteristics from a detected image until the average intensity difference between neighboring pixels in the detected image is maximally.

9. An analysis apparatus as claimed in claim 4, wherein said auto-focusing means are adapted for controlling the focusing means to change the focusing of the monitoring system, the excitation system and the detection system based on the determined image characteristics, for controlling the monitoring system to repeatedly image the target region and for controlling the image processing means to determine the image characteristics from a detected image until the absolute intensity difference between neighboring pixels in the detected image is maximally.

10. An analysis apparatus as claimed in claim 1, wherein the monitoring system is adapted for orthogonal polarized spectral imaging, in particular for bichromatic orthogonal polarized spectral imaging.

11. An analysis method, in particular a spectroscopic analysis method, for analyzing an object comprising the steps of:

emitting an excitation beam by an excitation system to excite a target region,

emitting a monitoring beam by a monitoring system to image the target region by an imaging system,

detecting scattered radiation from the target region generated by the excitation beam by a detection system,

focusing the excitation system, the monitoring system and the detection system on a detection plane in the target region by a focusing means,

determining image characteristics, which indicate if the imaging system is focused on the object to be analyzed, from a detected image, and

controlling the focusing means to change the focusing of the monitoring system, the excitation system and the detection system based on the determined image characteristics, controlling the monitoring system to image the target region, and controlling the image processing means to determine the image characteristics from a detected image until the object substantially lies in the detection plane.

12. An optical focusing system for focusing on a target point of an object, comprising:

a target system to be focused on the target point,

a monitoring system comprising a monitoring beam source for emitting a monitoring beam and an imaging system to image the target region,

focusing means for focusing the target system and the monitoring system on a detection plane in the target region,

image processing means for determining image characteristics, which indicate if the imaging system is focused on the object to be analyzed, from a detected image, and

auto-focusing means for controlling the focusing means to change the focusing of the monitoring system and the target system based on the determined image characteristics, for controlling the monitoring system to image the target region and for controlling the image processing means to determine the image characteristics from a detected image until the object substantially lies in the detection planed.

13. An optical tracking system as claimed in claim 12, wherein said target system comprises a light beam generation means for emitting a light beam, in particular a laser for emitting a laser beam, to be focused on the target point of the object.

14. An optical tracking system as claimed in claim 12, adapted for use in the field of laser surgery, laser cutting, laser welding, laser shaving, photodynamic therapy, radio therapy, remote sensing and target and tracking.