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Method for the specific identification of orthopoxvirus with the aid of a miniature biological chip

  • US 20060147905A1
  • Filed: 11/26/2001
  • Published: 07/06/2006
  • Est. Priority Date: 11/26/2001
  • Status: Abandoned Application
First Claim
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1. A method for the identification of virus species of the Orthopoxvirus genus by hybridizing an appropriately treated as described in step (b) orthopoxvirus DNA isolated from a test sample to a miniature biological chip, comprising the steps of (a) providing a biochip for species diagnostics in the form of a microarray of gel spots on a glass support with subsequent immobilization of typing oligonucleotides, (b) amplifying a fragment of the crmB gene by a two-step asymmetrical PCR using a fluorescently labeled primer to obtain single-stranded DNA for hybridization, (c) performing DNA-DNA hybridization on said biochip, whereby a hybridization mix is incubated in a sealed reaction chamber over the microchip allowing the duplex formation between said fragment of viral DNA and the corresponding complementary oligonucleotides on the chip, (d) performing detection and analysis of the hybridization results, whereby fluorescent signals are detected from the labeled sample using a recording device and the resulting hybridization pattern is subsequently compared to reference patterns that allow an adequate interpretation of the results.

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