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Novel probe and its use in bioaffinity assays

  • US 20060147954A1
  • Filed: 10/19/2005
  • Published: 07/06/2006
  • Est. Priority Date: 10/19/2004
  • Status: Abandoned Application
First Claim
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1. A homogeneous bioaffinity assay comprising the steps of a) providing a labelled probe comprising (i) a TR-FRET label pair comprising a long excited state lifetime luminescent energy donor and a short excited state lifetime luminescent energy acceptor;

  • and (ii) a reactive region, capable of recognizing a bioaffinity component to be determined;

    b) contacting said labelled probe with a sample, expected to comprise said bioaffinity component;

    c) allowing said probe and said bioaffinity component to undergo a recognition reaction;

    said probe having different energy transfer efficiency between the donor and the acceptor when reacted to said bioaffinity component as compared to non-reacted probe;

    d) measuring an energy transfer based signal from the acceptor of said reacted sample;

    e) measuring an energy transfer based signal from the acceptor of a non-reacted probe in a reference assay;

    f) comparing the signals obtained in steps d) and e); and

    g) determining the presence or absence of the bioaffinity component in the sample based on a difference in acceptor signals obtained in step (f).

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