Mycobacterial rpoB sequences
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Abstract
This invention provides polynucleotide probes, sequences and methods for speciating and phenotyping organisms, for example, using probes based on the Mycobacterium tuberculosis rpoB gene. The groups or species to which an organism belongs may be determined by comparing hybridization patterns of target nucleic acid from the organism to hybridization patterns in a database.
23 Citations
28 Claims
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1-19. -19. (canceled)
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20. A polynucleotide probe or primer that hybridizes under stringent hybridization conditions to a mycobacterial rpoB sequence selected from the group consisting of SEQ ID NOS:
- 2, 3, 4, 5, 6 and 9 or its complement without hybridizing to the M. tuberculosis sequence of SEQ ID NO;
1 or its complement, wherein said stringent hybridization conditions comprise 5×
SSPE and a temperature of 25-30°
C. - View Dependent Claims (21, 22, 23, 24, 25)
- 2, 3, 4, 5, 6 and 9 or its complement without hybridizing to the M. tuberculosis sequence of SEQ ID NO;
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26. A polynucleotide probe or primer that hybridizes under stringent hybridization conditions to at least 100 contiguous bases of a mycobacterial rpoB sequence selected from the group consisting of SEQ ID NOS:
- 8 and 10 or its complement without hybridizing to the M. tuberculosis sequence of SEQ ID NO;
1 or its complement, wherein said stringent hybridization conditions comprise 5×
SSPE and a temperature of 25-30°
C. - View Dependent Claims (27, 28)
- 8 and 10 or its complement without hybridizing to the M. tuberculosis sequence of SEQ ID NO;
Specification