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Methods for array preparation using substrate rotation

  • US 20060147981A1
  • Filed: 02/28/2006
  • Published: 07/06/2006
  • Est. Priority Date: 06/01/2000
  • Status: Abandoned Application
First Claim
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1. A method of preparing a nucleic acid array on a support, wherein each nucleic acid occupies a separate known region of the support, said synthesizing comprising:

  • (a) activating a region of the support;

    (b) attaching a nucleotide to a first region, said nucleotide having a masked reactive site linked to a protecting group;

    (c) repeating steps (a) and (b) on other regions of said support whereby each of said other regions has bound thereto another nucleotide comprising a masked reactive site link to a protecting group, wherein said another nucleotide may be the same or different from that used in step (b);

    (d) removing the protecting group from one of the nucleotides bound to one of the regions of the support to provide a region bearing a nucleotide having an unmasked reactive site;

    (e) binding an additional nucleotide to the nucleotide with an unmasked reactive site;

    (f) repeating steps (d) and (e) on regions of the support until a desired plurality of nucleic acids is synthesized, each nucleic acid occupying separate known regions of the support;

    wherein the surface of said substrate is maintained in a position which is vertical or within about 30 degrees of vertical, and wherein the substrate is rotated around an axis perpendicular to said surface by an amount of from about 20 degrees to about 180 degrees, said rotating being done prior to, coincident with or subsequent to at least one of said attaching or binding steps.

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