Assay for tissue factor and factor VIIa
First Claim
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1. A method for determining tissue factor (TF) activity in a sample suspected to contain TF, comprising:
- (a) combining TF and a molar excess of factor VIIa (fVIIa) to produce a TF/fVIIa enzyme complex; and
(b) detecting enzymatic activity of the complex using a fluorogenic or chromogenic substrate.
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Abstract
The invention provides assays for detecting and quantitating tissue factor and factor VIIa in simple and complex biological systems. The assays are performed by detecting and/or measuring the tissue factor cofactor activity and factor VIIa enzymatic activity using aminonapthalene-based fluorogenic substrates.
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Citations
29 Claims
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1. A method for determining tissue factor (TF) activity in a sample suspected to contain TF, comprising:
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(a) combining TF and a molar excess of factor VIIa (fVIIa) to produce a TF/fVIIa enzyme complex; and
(b) detecting enzymatic activity of the complex using a fluorogenic or chromogenic substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method for determining factor VIIa (fVIIa) enzymatic activity in a sample suspected to contain fVIIa, comprising:
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(a) combining fVIIa and a molar excess of TF to produce a TF/fVIIa enzyme complex; and
(b) detecting the enzymatic activity of the complex using a fluorogenic substrate or a chromogenic substrate. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29)
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Specification