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Metabolic biosensor and uses thereof

  • US 20060154326A1
  • Filed: 12/19/2005
  • Published: 07/13/2006
  • Est. Priority Date: 06/07/2001
  • Status: Abandoned Application
First Claim
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1. A device for measuring a substrate as an analysis of the metabolism of a cell in a culture medium comprising;

  • a first reaction vessel comprising a first oxidation or reduction reaction mixture containing a first enzyme and a cofactor, said first enzyme being capable of chemically reacting with the substrate to be measured, producing a product and causing reduction or oxidation of the cofactor;

    a second reaction vessel containing a second oxidation or reduction reaction mixture containing a second enzyme, said second enzyme being capable of chemically further reacting with the product produced in the first reaction vessel producing a second product and causing reduction or oxidation of the cofactor;

    a detector for determination of the cofactor reduced or oxidized in the first and/or second reaction vessel, said first and second reaction vessels and said detector being in fluid connection together in a closed circuit.

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