Multidimensional liquid chromatography/spectrometry
First Claim
1. A liquid chromatograph spectrometer comprising:
- a) a first liquid chromatography (LC) column comprising a first resin connected in tandem to a second LC column having an outlet and comprising a second resin;
b) a third LC column having an outlet and an inlet, and comprising a third resin; and
c) a spectrometer operably connected to the third column outlet;
wherein the first, second and third resins have distinct separation characteristics, and wherein the third LC column inlet is operably connected to the second LC column outlet.
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Accused Products
Abstract
A liquid chromatography configuration providing at least three dimensions of separation coupled with spectrometry greatly improves the ability to detect ions present in samples, including complex biological samples such as blood. Liquid chromatography columns in one embodiment are connected with an in-line trapping column the alternately communicates with the second and third liquid chromatography columns. The liquid chromatography columns are operably connected either to a mass spectrometer (MS) or a nuclear magnetic resonance (NMR) spectrometer. The improved dynamic range of detection allows a method of detecting molecular components present in complex biological samples that serve as biomarkers for a disease state, such as sepsis.
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Citations
41 Claims
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1. A liquid chromatograph spectrometer comprising:
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a) a first liquid chromatography (LC) column comprising a first resin connected in tandem to a second LC column having an outlet and comprising a second resin;
b) a third LC column having an outlet and an inlet, and comprising a third resin; and
c) a spectrometer operably connected to the third column outlet;
wherein the first, second and third resins have distinct separation characteristics, and wherein the third LC column inlet is operably connected to the second LC column outlet. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method of detecting a molecular component of a sample, comprising:
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a) fractionating a sample with a first liquid chromatography (LC) column comprising a first resin connected in tandem to a second LC column comprising a second resin;
b) loading a fraction of molecules eluted from the second column onto a third LC column comprising a third resin that is operably connected to the second column;
c) eluting a fraction of the molecules loaded onto the third column; and
d) analyzing the fraction with a spectrometer that is operably linked to the third column, wherein the first, second and third resins have distinct separation characteristics, and wherein the analysis provides detection of at least one molecular component of the sample. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41)
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Specification