Use of flavivirus for the expression of protein epitopes and development of new live attenuated vaccine virus to immunize against flavivirus and other infectious agents
First Claim
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1. A method for the production of Flavivirus as a vector for heterologous antigens comprising the introduction and expression of foreign gene sequences into insertion sites at the level of the envelope protein of any Flavivirus, wherein the sites are structurally apart from areas known to interfere with the overall flavivirus E protein structure and comprising:
- (i) sites that lie on the external surface of the virus providing accessibility to antibody;
(ii) not disrupt or significantly destabilize the three-dimensional structure of the E protein and (iii) not interfere with the formation of the E protein network within the viral envelope.
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Abstract
The present invention relates to a vaccine against infections caused by flavivirus. More particularly to the use of the YF vaccine virus (17D) to express at the level of its envelope, protein epitopes from other pathogens which will elicit a specific immune response to the parental pathogen.
18 Citations
58 Claims
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1. A method for the production of Flavivirus as a vector for heterologous antigens comprising the introduction and expression of foreign gene sequences into insertion sites at the level of the envelope protein of any Flavivirus, wherein the sites are structurally apart from areas known to interfere with the overall flavivirus E protein structure and comprising:
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(i) sites that lie on the external surface of the virus providing accessibility to antibody;
(ii) not disrupt or significantly destabilize the three-dimensional structure of the E protein and (iii) not interfere with the formation of the E protein network within the viral envelope. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 58)
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33. DNA construct having the structure of plasmid pYFE200.
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34. DNA construct having the structure of plasmid pYFE200/1.
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35. DNA construct having the structure of plasmid pYFE200/13.
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36. DNA construct having the structure of plasmid pYFE200/8.
- 37. A Flavivirus as a vector for heterologous antigens comprising foreign gene sequences inserted at sites in the level of its envelope protein, wherein the sites are structurally apart from areas known to interfere with the overall flavivirus E protein structure.
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