Multi-layer collagenic article useful for wounds healing and a method for its production thereof

• US 20060159731A1
• Filed: 12/20/2005
• Published: 07/20/2006
• Est. Priority Date: 06/03/2002
• Status: Abandoned Application

• Current Assignee: Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. (Hebrew University of Jerusalem)
• Original Assignee: Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. (Hebrew University of Jerusalem)
• Inventors: Shoshan, Shmuel
• Application Number:
  US11/311,299
• Publication Number:
  US 20060159731A1
• Time in Patent Office: Days
• US Class Current: 424/443
• CPC Class Codes: A61K 38/39 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
• CPC Class Codes: A61L 15/325 Collagen

Abstract

A multi-layer collagen article useful for wound healing includes at least two layers; wherein at least one layer, facing the wound side, has an effective amount of non or partially cross-linked collagen; and at least one layer having an effective amount of highly cross-linked collagen matrices. A method for the production of the collagen article and a method of enhancing wound healing by means of administering the multi-layer collagen are provided.

Claims

1. A multi-layer collagen article useful for wound healing, comprising at least two layers;

wherein at least one layer, facing the wound side, is comprising an effective amount of non or partially cross-linked collagen; and

at least one layer comprising an effective amount of highly cross-linked collagen matrices.

2. A multi-layer collagen article useful for wound healing as claim 1, wherein said multi-layer wound healing dressing comprising at least one sponge collagen matrix.

3. A multi-layer collagen article useful for wound healing as claim 1, wherein said a multi-layer wound healing dressing comprising at least one thin membranal collagen sheet.

4. A multi-layer collagen article according to claim 1 wherein the non or partially crossed linked collagen is recombinant human collagen.

5. A multi layer collagen article according to claim 4 wherein the recombinant human collagen is monomeric

6. A method for the production of collagen article, as defined in one of the previous claims, comprising but not limited to the operations of preparing non-crosslinked collagens;

non-enzymatic glycosylating said matrices;

integrating the layers by means of thermally reconstituting said formed collagen fibers by monosaccharide-aldehyde;

washing and lyophilizing said formed crossed-linked layer, and dressing a wound, wherein the smooth surface of the collagen non or partially crossed-linked collagen layer is facing the surface of said wound.

7. A method of enhancing wound healing, by means of administrating a multi-layer collagen, as defined in claim 1.

8. A method according to claim 7, wherein said collagen wound healing dressing administrated onto wounds, cuts or burns in dermal or dental injured tissues.