Method of providing hemostasis to a wound

• US 20060159733A1
• Filed: 02/23/2006
• Published: 07/20/2006
• Est. Priority Date: 11/26/2002
• Status: Abandoned Application

• Current Assignee: Ethicon Incorporated (Johnson & Johnson)
• Original Assignee: Ethicon Incorporated (Johnson & Johnson)
• Inventors: Pendharkar, Sanyog M., Gorman, Anne J.
• Application Number:
  US11/360,864
• Publication Number:
  US 20060159733A1
• Time in Patent Office: Days
• US Class Current: 424/445
• CPC Class Codes: A61K 31/715 Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
• CPC Class Codes: A61K 31/717 Celluloses
• CPC Class Codes: A61K 38/095 Oxytocins; Vasopressins; Related peptides
• CPC Class Codes: A61K 38/363 Fibrinogen
• CPC Class Codes: A61K 38/38 Albumins
• CPC Class Codes: A61K 38/39 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
• CPC Class Codes: A61K 38/4833 Thrombin (3.4.21.5)
• CPC Class Codes: A61K 38/4846 Factor VII (3.4.21.21); Factor IX (3.4.21.22); Factor Xa (3.4.21.6); Factor XI (3.4.21.27); Factor XII (3.4.21.38)
• CPC Class Codes: A61L 15/28 Polysaccharides or their derivatives A61L15/225 takes precedence
• CPC Class Codes: A61L 15/44 Medicaments
• CPC Class Codes: A61L 2300/418 Agents promoting blood coagulation, blood-clotting agents, embolising agents
• CPC Class Codes: A61L 2400/04 Materials for stopping bleeding
• CPC Class Codes: C08L 1/00 Compositions of cellulose, modified cellulose or cellulose derivatives
• CPC Class Codes: C08L 1/04 Oxycellulose; Hydrocellulose , e.g. microcrystalline cellulose
• CPC Class Codes: C08L 5/00 Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00

Abstract

The present invention is directed to hemostatic wound dressings that contain a substrate for contacting a wound, wherein the substrate includes a wound-contacting surface and is fabricated at least in part from a biocompatible aldehyde-modified polysaccharide having covalently conjugated there with a hemostatic agent and to methods of providing hemostasis to a wound that include applying the wound dressing described herein to a wound.

Claims

1. A method of providing hemostasis to a wound, comprising:

applying to a wound a dressing comprising a biocompatible, aldehyde-modified, regenerated polysaccharide

comprising repeating units of structure I

where x and y represent mole percent, x plus y equals 100 percent, x is from about 95 to about 5, y is from about 5 to about 95;

R is selected from the group consisting of CH₂OR₃, COOR₄, sulphonic acid and phosphonic acid, R₃ and R₄ are selected from the group consisting of H, alkyl aryl, alkoxy and aryloxy, and R₁ and R₂ are selected from the groups consisting of H, alkyl, aryl, alkoxy, aryloxy, sulphonyl and phosphoryl.

2. The method of claim 1 wherein said dressing comprises a fiber, a fabric, a sponge, a foam, a film, a bead, a gel, a powder, or combinations thereof

3. The method of claim 1 wherein said aldehyde-modified regenerated polysaccharide is selected from the group consisting of aldehyde-modified regenerated cellulose, alkyl cellulose, hydroxyalkyl cellulose, alkylhydroxyalkyl cellulose, cellulose sulfate, salts of carboxymethyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose, chitin, carboxymethyl chitin, hyaluronic acid, salts of hyaluronic acid, alginate, alginic acid, propylene glycol alginate, glycogen, dextran, dextran sulfate, curdlan, pectin, pullulan, xanthan, chondroitin, chondroitin sulfates, carboxymethyl dextran, carboxymethyl chitosan, chitosan, heparin, heparin sulfate, heparin sulfate, dermatan sulfate, keratin sulfate, carrageenans, chitosan, starch, amylose, amylopectin, poly-N-glucosamine, polymannuronic acid, polyglucuronic acid, polyguluronic acid and derivatives of the above.

4. The method of claim 3 wherein said aldehyde-modified regenerated polysaccharide comprises an amount of aldehyde effective to render the polysaccharide biodegradable.

5. The wound dressing of claim 4 wherein said aldehyde-modified regenerated cellulose comprises repeating units of structure II,

wherein x plus y equals 100 percent, x ranges from about 95 to about 5 percent, and y ranges from about 5 to about 95 percent and R is CH₂OH, and R₁ and R₂ are H.

6. The method of claim 5 wherein x ranges from about 80 to about 20 percent and y ranges from about 20 to about 80 percent.

7. The method of claim 1 wherein said aldehyde-modified polysaccharide is essentially free of carboxylic acid.

8. The method of claim 5 wherein said aldehyde-modified cellulose is essentially free of carboxylic acid.

9. The wound of claim 1 wherein said dressing further comprises a synthetic, recombinant or naturally occurring hemostatic agent covalently conjugated with said aldehyde-modified regenerated polysaccharide, said agent comprising an aldehyde-reactive moiety.

10. The method of claim 9 wherein said hemostatic agent is selected from the group consisting prothrombin, thrombin, fibrinogen, fibrin, fibronectin, heparinase, Factor X/Xa, Factor VII/VIIa, Factor IX/IXa, Factor XI/XIa, Factor XII/XIIa, tissue factor, batroxobin, ancrod, ecarin, von Willebrand Factor, collagen, elastin, albumin, gelatin, platelet surface glycoproteins, vasopressin, vasopressin analogs, epinephrine, selectin, procoagulant venom, plasminogen activator inhibitor, platelet activating agents and synthetic peptides having hemostatic activity.

11. The method of claim 10 wherein said dressing comprises from about 0.001 to about 50 percent by weight of said hemostatic agent.

12. The method of claim 10 wherein said dressing comprises from about 0.001 to about 1 percent by weight of thrombin as the hemostatic agent.

13. The method of claim 10 wherein said dressing comprises from about 0.1 to about 50 percent by weight of fibrinogen as the hemostatic agent.