Labelled nucleotides
First Claim
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1. A nucleotide or nucleoside molecule, having a base that is linked to a detectable label via a cleavable linker.
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Abstract
Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group.
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Citations
25 Claims
- 1. A nucleotide or nucleoside molecule, having a base that is linked to a detectable label via a cleavable linker.
- 9. A method of labeling a nucleic acid molecule, the method comprising incorporating into the nucleic acid molecule a nucleotide or nucleoside molecule, wherein the nucleotide or nucleoside molecule has a base that is linked to a detectable label via a cleavable linker.
- 17. A method for determining the sequence of a target single-stranded polynucleotide, comprising monitoring the sequential incorporation of complementary nucleotides, wherein the nucleotides each have a base that is linked to a detectable label via a cleavable linker, and wherein the identity of each nucleotide incorporated is determined by detection of the label linked to the base, and subsequent removal of the label.
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18. A method for determining the sequence of a target single-stranded polynucleotide, comprising:
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(a) providing nucleotides, wherein the nucleotides have a base that is linked to a detectable label via a cleavable linker, and wherein the detectable label linked to each type of nucleotide can be distinguished upon detection from the detectable label used for other types of nucleotides;
(b) incorporating a nucleotide into the complement of the target single stranded polynucleotide;
(c) detecting the label of the nucleotide of (b), thereby determining the type of nucleotide incorporated;
(d) removing the label of the nucleotide of (b); and
(e) optionally repeating steps (b)-(d) one or more times;
thereby determining the sequence of a target single-stranded polynucleotide.
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24. A kit, comprising:
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(c) individual nucleotides, wherein each nucleotide has a base that is linked to a detectable label via a cleavable linker, and wherein the detectable label linked to each nucleotide can be distinguished upon detection from the detectable label used for other three nucleotides; and
(b) packaging materials therefor.
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Specification