Lateral flow assay device and method
First Claim
1. A lateral flow assay device to test for the presence and/or amount of a nucleic acid sequence of interest in a sample comprising:
- (a) a sample receiving zone for contacting the device with a sample to be tested;
(b) an extraction zone for extraction of nucleic acid from the sample;
(c) a nucleic acid amplification zone in liquid communication with the sample receiving zone; and
(d) a detection zone for detecting the product/s, directly or indirectly, of a nucleic acid amplification reaction performed in the nucleic acid amplification zone, said detection zone being in liquid communication with the amplification zone;
the device also comprising a porous matrix which, at a proximal end, is in liquid communication with the sample receiving zone such that liquid applied to the sample receiving zone flows along the device through the porous matrix by capillary action.
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Accused Products
Abstract
Disclosed is a lateral flow assay device to test for the presence and/or amount of a nucleic acid sequence of interest in a sample, the lateral flow device comprising: (a) a sample receiving zone for contacting the device with a sample to be tested; (b) an extraction zone (8) for extraction of nucleic acid from the sample; (c) a nucleic acid amplification zone (18) in liquid communication with the sample receiving zone; and (d) a detection zone (20) for detecting the products, directly or indirectly, of a nucleic acid amplification reaction performed in the nucleic acid amplification zone, said detection zone (20) being, or being locatable, in liquid communication with the amplification zone (18).
77 Citations
44 Claims
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1. A lateral flow assay device to test for the presence and/or amount of a nucleic acid sequence of interest in a sample comprising:
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(a) a sample receiving zone for contacting the device with a sample to be tested;
(b) an extraction zone for extraction of nucleic acid from the sample;
(c) a nucleic acid amplification zone in liquid communication with the sample receiving zone; and
(d) a detection zone for detecting the product/s, directly or indirectly, of a nucleic acid amplification reaction performed in the nucleic acid amplification zone, said detection zone being in liquid communication with the amplification zone;
the device also comprising a porous matrix which, at a proximal end, is in liquid communication with the sample receiving zone such that liquid applied to the sample receiving zone flows along the device through the porous matrix by capillary action. - View Dependent Claims (2, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44)
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3-26. -26. (canceled)
Specification