Fab library for the preparation of anti VEGF and anti rabies virus fabs
First Claim
1. A method for producing a composition comprising at least two different proteinaceous molecules comprising paired variable regions, wherein the at least two proteinaceous molecules have different binding specificities, said method comprising:
- contacting at least three different variable regions under conditions allowing for pairing of variable regions, and harvesting essentially all proteinaceous molecules having binding specificities resulting from said pairing, so as to produce said composition.
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Accused Products
Abstract
The present invention provides combinations of specific binding proteins, such as immunoglobulins, that are designed to be true combinations, essentially all components of the combination being functional and compatible with each other. The invention further provides a method for producing a composition comprising at least two different proteinaceous molecules comprising paired variable regions, the at least two proteinaceous molecules having different binding specificities, comprising paired variable regions, at least two proteinaceous molecules having different binding specificities, comprising contacting at least three different variable regions under conditions allowing for pairing of variable regions and harvesting essentially all proteinaceous molecules having binding specificities resulting from the pairing.
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Citations
43 Claims
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1. A method for producing a composition comprising at least two different proteinaceous molecules comprising paired variable regions, wherein the at least two proteinaceous molecules have different binding specificities, said method comprising:
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contacting at least three different variable regions under conditions allowing for pairing of variable regions, and harvesting essentially all proteinaceous molecules having binding specificities resulting from said pairing, so as to produce said composition. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 34, 35, 36, 37, 39, 40, 41, 42, 43)
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33. (canceled)
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38. A method for producing nucleic acid sequences encoding variable regions for use in a process for producing a composition comprising at least two different proteinaceous molecules comprising paired variable regions, wherein the at least two proteinaceous molecules have different binding specificities, said method comprising:
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a combination of;
synthesizing nucleic acid sequences encoding variable regions, expressing said nucleic acid sequences and allowing the expression products to pair, and selecting nucleic acid sequences encoding variable regions having desired pairing behavior, with altering existing nucleic acid sequences encoding variable regions, expressing said nucleic acid sequences, and allowing the expression products to pair and selecting nucleic acid sequences encoding variable regions having desired pairing behavior.
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Specification