In vitro and in vivo assessment of organs and tissue and use, transplant, freshness and tissue conditions

• US 20060161073A1
• Filed: 03/18/2006
• Published: 07/20/2006
• Est. Priority Date: 05/03/2001
• Status: Abandoned Application

• Current Assignee: John D. Kutzko, Michael G. Singer
• Original Assignee: John D. Kutzko, Michael G. Singer
• Inventors: Kutzko, John D., Singer, Michael G.
• Application Number:
  US11/386,016
• Publication Number:
  US 20060161073A1
• Time in Patent Office: Days
• US Class Current: 600/547
• CPC Class Codes: A61B 2017/00969 used for transplantation
• CPC Class Codes: A61B 5/0537 Measuring body composition by impedance, e.g. tissue hydration or fat content
• CPC Class Codes: A61B 5/413 Monitoring transplanted tissue or organ, e.g. for possible rejection reactions after a transplant
• CPC Class Codes: A61B 5/416 the spleen
• CPC Class Codes: A61B 5/4869 Determining body composition

Abstract

A method of organ and tissue vitality assessment in a biological entity, human, animal, fruit or vegetable, including the steps of: utilizing bioelectric impedance analysis in a biological model for composition analysis; and using the results of the utilizing step to provide an objective assessment of volume and distribution of fluid and tissues, and electrical health of cells and membranes of the organ or tissue.

Claims

1. A method of organ and tissue vitality assessment in a biological entity, human, animal, fruit or vegetable, comprising the steps of:

utilizing bioelectric impedance analysis in a biological model for composition analysis; and

using the results of said utilizing step to provide an objective assessment of volume and distribution of fluid and tissues, as well as electrical health of cells and membranes of said organ or tissue of any biological entity.

2. The method according to claim 1, including the step of:

utilizing a modified bioelectric impedance analysis for composition analysis to assess the health of cells of said organs and tissues or the biological entity by the measured reactance thereof.

3. A method according to claim 1, wherein:

upon harvesting, treating or transporting said organ or tissue or meat, fish, fruit or vegetable from the donor or source, including the steps of;

placing signal introduction electrodes on opposite lateral peripheral borders of said organ tissue, meat, fish, fruit or vegetable;

placing signal detection electrodes at superior and inferior borders of said organ tissue, meat, fish, fruit or vegetable for a first part of an initial measurement;

measuring and recording first values of resistance and reactance and calculating the phase angle of said organ tissue, meat, fish, fruit or vegetable in said initial measurement;

then placing said signal introduction electrodes on said superior and said inferior borders of said organ tissue, meat, fish, fruit or vegetable;

placing said signal detection electrodes on said opposite lateral borders of said organ tissue, meat, fish, fruit or vegetable;

measuring and recording second values of said resistance and said reactance and calculating the phase angle of said organ tissue, meat, fish, fruit or vegetable; and

comparing said first and second values to normal values to assess vitality.

4. A method according to claim 2, wherein:

upon arrival of said organ tissue, meat, fish, fruit or vegetable at the location of the recipient including the steps of;

placing signal introduction electrodes on opposite lateral peripheral borders of said organ, tissue, meat, fish, fruit or vegetable;

placing signal detection electrodes at superior and inferior borders of said organ, tissue, meat, fish, fruit or vegetable for a first part of an initial measurement of said organ, tissue, meat, fish, fruit or vegetable;

measuring and recording first values of resistance and reactance and calculating the phase angle of said organ, tissue, meat, fish, fruit or vegetable in said initial measurement;

then placing said signal introduction electrodes on said superior and said inferior borders of said organ, tissue, meat, fish, fruit or vegetable;

placing said signal detection electrodes on said opposite lateral borders of said organ, tissue, meat, fish, fruit or vegetable;

measuring and recording second values of said resistance and said reactance and calculating the phase angle of said organ, tissue, meat, fish, fruit or vegetable; and

comparing said first and second values to normal values to assess vitality of said organ, tissue, meat, fish, fruit or vegetable.

5. A method according to claim 3, wherein:

prior to the implantation, treatment and/or consumption of said organ tissue, meat, fish, fruit or vegetable into the recipient including the following additional steps;

again placing said signal introduction electrodes on said opposite lateral peripheral borders of said organ tissue, meat, fish, fruit or vegetable;

again placing said signal detection electrodes at said superior and said inferior borders of said organ tissue, meat, fish, fruit or vegetable;

measuring and recording third values of resistance and reactance and calculating the phase angle of said organ tissue, meat, fish, fruit or vegetable;

then again placing said signal introduction electrodes on said superior and said inferior borders of said organ tissue, meat, fish, fruit or vegetable;

again placing said signal detection electrodes on said opposite lateral borders of said organ tissue, meat, fish, fruit or vegetable;

measuring and recording fourth values of said resistance and said reactance of said organ tissue, meat, fish, fruit or vegetable; and

comparing said first and second values to said third and fourth values to determine if the values are within a predetermined acceptable range of agreement denoting no further loss of said organ tissue, meat, fish, fruit or vegetable vitality.

6. A method according to claim 4, including the following additional steps:

again placing said signal introduction electrodes on said opposite lateral peripheral borders of said organ tissue, meat, fish, fruit or vegetable;

again placing said signal detection electrodes at said superior and said inferior borders of said organ tissue, meat, fish, fruit or vegetable;

measuring and recording third values of resistance and reactance and calculating the phase angle of said organ tissue, meat, fish, fruit or vegetable;

then again placing said signal introduction electrodes on said superior and said inferior borders of said organ tissue, meat, fish, fruit or vegetable;

again placing said signal detection electrodes on said opposite lateral borders of said organ tissue, meat, fish, fruit or vegetable;

measuring and recording fourth values of said resistance and said reactance and calculating the phase angle of said organ tissue, meat, fish, fruit or vegetable; and

comparing said first and second values to said third and fourth values to determine if the values are within a predetermined acceptable range of agreement denoting no further loss of said organ tissue, meat, fish, fruit or vegetable vitality.

13. A method according to claim 3, wherein:

said measured values of resistance and reactance and the calculation of phase angle changes will be compared to their previous values and considered in the rate of change either increase or decrease the assessment of fluid volumes, cellular architecture, freshness and vitality.

14. A method according to claim 3, including the steps of:

comparing and assessing homogeneity within heterogeneous populations based upon comparative values of calculated phase angles.

15. A method according to claim 3, wherein:

the severity, criticality or burden of an adverse condition is based upon said calculated phase angle value in that a higher value indicates a less severe, critical or burden of adversity and a lower value indicates a greater severity, criticality or burden of adversity.

16. A method according to claim 15 wherein:

the resources allocated or required to manage said adverse condition are based upon said calculated phase angle value in that the lower phase angle value entity requires greater resources than that of an entity with a greater phase angle value.

17. A method according to claim 16, wherein:

in those entities that experience a transient reduction of said phase angle value that does not fully return to the previous baseline phase angle value after apparent recovery that that entity is not fully recovered and may be predisposed to further adversity and require additional care and intervention.

19. A method according to claim 3, wherein:

the freshness of a consumable biological foodstuff such as a meat, fish, fowl, fruit or vegetable is based upon said calculated phase angle value in which the higher said phase angle value as related to that value upon initial harvest or processing is compared to that level after transport or upon purchase or process for purchase.

20. A method according to claim 19, wherein:

said phase angle is used as a freshness indicator of said consumable biological foodstuff.

7. A method of organ, tissue, meat, fish, fruit or vegetable vitality assessment, comprising the steps of:

placing signal introduction electrodes on opposite lateral peripheral borders of said organ, tissue, meat, fish, fruit or vegetable;

placing signal detection electrodes at superior and inferior borders of said organ, tissue, meat, fish, fruit or vegetable for a first part of an initial measurement of said organ tissue, meat, fish, fruit or vegetable;

measuring and recording first values of resistance and reactance of said organ, tissue, meat, fish, fruit or vegetable in said initial measurement;

then placing said signal introduction electrodes on said superior and said inferior borders of said organ tissue, meat, fish, fruit or vegetable;

placing said signal detection electrodes on said opposite lateral borders of said organ, tissue, meat, fish, fruit or vegetable;

measuring and recording second values of said resistance and said reactance of said organ, tissue, meat, fish, fruit or vegetable; and

comparing said first and second values to normal values to assess vitality of said organ, tissue, meat, fish, fruit or vegetable.

8. A method according to claim 7, including the following additional steps:

again placing said signal introduction electrodes on said opposite lateral peripheral borders of said organ, tissue, meat, fish, fruit or vegetable;

again placing said signal detection electrodes at said superior and said inferior borders of said organ, tissue, meat, fish, fruit or vegetable;

measuring and recording third values of resistance and reactance and calculating the phase angle of said organ, tissue, meat, fish, fruit or vegetable;

then again placing said signal introduction electrodes on said superior and said inferior borders of said organ, tissue, meat, fish, fruit or vegetable;

again placing said signal detection electrodes on said opposite lateral borders of said organ, tissue, meat, fish, fruit or vegetable;

measuring and recording fourth values of said resistance and said reactance and calculating the phase angle of said organ, tissue, meat, fish, fruit or vegetable; and

comparing said first and second values to said third and fourth values to determine if the values are within a predetermined acceptable range of agreement denoting no further loss of said organ, tissue, meat, fish, fruit or vegetable vitality.

9. A method of organ, tissue, meat, fish, fruit or vegetable vitality assessment for transplantation, treatment, consumption or transport of an organ, tissue, meat, fish, fruit or vegetable being assessed, comprising the steps of:

placing signal introduction electrodes on opposite lateral peripheral borders of said organ, tissue, meat, fish, fruit or vegetable upon harvesting of said organ tissue, meat, fish, fruit or vegetable;

placing signal detection electrodes at superior and inferior borders of said organ tissue, meat, fish, fruit or vegetable for a first part of an initial measurement upon said harvesting of said organ, tissue, meat, fish, fruit or vegetable;

measuring and recording first values of resistance and reactance of said organ, tissue, meat, fish, fruit or vegetable in said initial measurement;

then placing said signal introduction electrodes on said superior and said inferior borders of said organ, tissue, meat, fish, fruit or vegetable;

placing said signal detection electrodes on said opposite lateral borders of said organ, tissue, meat, fish, fruit or vegetable;

measuring and recording second values of said resistance and said reactance of said organ, tissue, meat, fish, fruit or vegetable; and

comparing said first and second values to normal values to determine if said organ, tissue, meat, fish, fruit or vegetable is acceptable or not for said transplantation, consumption, treatment or transportation effects.

10. A method according to claim 9, wherein:

if said organ, tissue, meat, fish, fruit or vegetable is acceptable, then prior to implanting, consuming or further treatment said organ, tissue, meat, fish, fruit or vegetable, performing the following steps;

again placing said signal introduction electrodes on said opposite lateral peripheral borders of said organ, tissue, meat, fish, fruit or vegetable;

again placing said signal detection electrodes at said superior and said inferior borders of said organ, tissue, meat, fish, fruit or vegetable for a first part of an initial post-harvest (transport/treatment)/pre-implant consumption, treatment or transport measurement;

measuring and recording third values of resistance and reactance of said organ, tissue, meat, fish, fruit or vegetable in said initial post-harvest (transport, treatment)/pre-implant measurement;

then placing said signal introduction electrodes on said superior and said inferior borders of said organ, tissue, meat, fish, fruit or vegetable;

placing said signal detection electrodes on said opposite lateral borders of said organ, tissue, meat, fish, fruit or vegetable;

measuring and recording fourth values of said resistance and said reactance of said organ, tissue, meat, fish, fruit or vegetable; and

comparing said first and second values to said third and fourth values to determine if the values are within a predetermined acceptable range of agreement denoting no further loss of said organ, tissue, meat, fish, fruit or vegetable vitality.

11. A method according to claim 9, including:

harvesting said organ from a first species of biological entity; and

implanting said organ in a different species of biological entity.

12. A method according to claim 10, including:

harvesting said organ from a first species of biological entity; and

implanting said organ in a different species of biological entity.

18. A method according to claim 10, wherein:

the vitality of said organ will have different levels of vitality based upon its measured resistance, reactance and calculated phase angle which while it may not be optimal will be sufficient for its purpose and may further be used to classify its use for a corresponding recipient with the matching of a higher phase angle value to the recipient with a lower phase angle value and conversely the matching of a organ with a lower phase angle value with a recipient of a higher phase angle value.